Recombinant Protein Expression Service

E.coli Expression System

MedChemExpress (MCE) offers a one-stop, customized E.coli protein expression service, including codon optimization, gene synthesis and subcloning, small-scale expression testing, amplified expression and purification, quality control, and delivery. We have an established protein refolding platform to recover and refold inclusion bodies into soluble proteins with biological activity. The overall success rate of our E.coli expression system exceeds 98%. Customers can flexibly choose options that meet their needs, experiencing unparalleled high-quality and competitively priced E.coli protein expression services.

Advantages of the E.coli expression system

01
Has a transparent
genetic background
and a relatively simple
structure.
02
Short turnaround
time and high cell
density.
03
High protein
expression level and
easy to amplify.
04
A rich selection of
expression vectors
and fusion tags.
05
Easy manipulation
to achieve optimal
expression.

Service Process

	Process	Description	Deliverable	Lead Time
1	Gene Synthesis, Protein Construct Design	Codon optimization; gene synthesis. Subcloning into appropriate expression vector. Transform plasmids into DH5α or TOP10 competent cells. Plasmid sequencing and preparation.	Purified protein QC COA /Project report	1-2 weeks
2	Protein Expression Evaluation, Optimization	Transform plasmids into host cells, e.g. BL21(DE3), BL21(DE3)pLysS, Rossetta(DE3), SHuffle T7, etc.. Select a single colony for small-scale induced expression. Determine optimal expression conditions.		1 week
3	Amplified expression and purification	Scale up protein expression using optimized conditions. Multiple-methods purification to obtain sufficient amounts of high-purity soluble proteins.		1-2 weeks
4	QC & Delivery	Purity analysis by SDS-PAGE WB/HPLC detection (Optional) Bradford assay for quantitation Provide COA/report		1 week

Bulk and Custom Product Services

Each project of recombinant protein customization requires evaluation to determine the corresponding plan and price. For further information on service prices or technical details, please send an email to sales@MedChemExpress.com Or call 609-228-6898 to contact us directly.

Salutation:		* Country or Region:
* Applicant Name:		* Organization Name:
* Department:		* Email Address:
* Phone Number
* Product Name:		* Requested quantity：
* Species:		* Source:
*Accession:		* Purity:
* Protein Application：
MCE Japan Authorized Agent:	ナミキ商事株式会社コスモ・バイオ株式会社
Remarks:

E.coli Cell-Free Expression System

E.coli Cell-Free Protein Synthesis System (CFPS) is a new protein preparation system that is different from traditional expression systems. It is a transcription/translation system based on extracts. By adding exogenous target mRNA or DNA templates, utilizing ribosomes, RNA polymerase, ATP and other substances in the cell extracts, and continuously adding some raw materials needed for protein synthesis such as amino acids, nucleotides, salt ions, etc., it synthesizes proteins in an extracellular environment without relying on living cells.

Advantages of the expression system

01
No need for cell culture or transfection; efficient and convenient.
02
Reaction conditions are controllable, open and flexible.
03
Reaction system is easy to scale up, enabling large-scale preparation.

Service Process

	Process	Description	Deliverable	Lead Time
1	Gene Synthesis, Protein Construct Design	Codon optimization; gene synthesis. Subcloning into pET series, pCold series and pBV220 vectors. Plasmid sequencing and preparation.	Purified protein QC COA /Project report	1-2 weeks
2	Protein Expression Evaluation, Optimization	Simultaneously detect the protein expression under different vectors and reaction conditions. Determine optimal expression conditions.		1 week
3	Amplified expression and purification	Scale up protein expression using optimized conditions. Multiple-methods purification to obtain sufficient amounts of high-purity soluble proteins.		1-2 weeks
4	QC & Delivery	Purity analysis by SDS-PAGE WB/HPLC detection (Optional) Bradford assay for quantitation Provide COA/report		1 week

Bulk and Custom Product Services

Each project of recombinant protein customization requires evaluation to determine the corresponding plan and price. For further information on service prices or technical details, please send an email to sales@MedChemExpress.com Or call 609-228-6898 to contact us directly.

Salutation:		* Country or Region:
* Applicant Name:		* Organization Name:
* Department:		* Email Address:
* Phone Number
* Product Name:		* Requested quantity：
* Species:		* Source:
*Accession:		* Purity:
* Protein Application：
MCE Japan Authorized Agent:	ナミキ商事株式会社コスモ・バイオ株式会社
Remarks:

Yeast Expression System

The yeast expression system mainly uses Saccharomyces cerevisiae and Pichia pastoris. Pichia pastoris is a rapidly developing and widely used eukaryotic expression system. It has two main advantages. On one hand, protein post-translational modifications can occur in yeast, which has a lower cost than mammalian expression systems. On the other hand, the heterologous proteins it expresses are first produced inside yeast cells and then secreted into the extracellular matrix through the yeast's secretion system, resulting in high-purity proteins that have undergone folding and modifications.

Advantages of the expression system

01
Has the post-translational modification function of
eukaryotic cells.
02
Fast growth rate and
capable of high-
density culture.
03
High level of protein
expression, conducive
to purification.
04
Can strictly regulate
the expression of
exogenous genes.

Service Process

	Process	Description	Deliverable	Lead Time
1	Gene Synthesis, Protein Construct Design	Codon optimization; gene synthesis. Subcloning into an appropriate expression vector, e.g.: pPIC9K, pPIC3.5K, etc.. Transform plasmids into DH5α or TOP10 competent cells. Plasmid sequencing and preparation.	Purified protein QC COA /Project report	1-2 weeks
2	Protein Expression Evaluation, Optimization	Linearize the plasmid and transform plasmids into host cells, e.g.: GS115, SMD1168, KM71, etc. screening positive colonies for small-scale induced expression. Determine optimal expression conditions.		2-3 weeks
3	Amplified expression and purification	Scale up protein expression using optimized conditions. Multiple-methods purification to obtain sufficient amounts of high-purity soluble proteins.		2-3 weeks
4	QC & Delivery	Purity analysis by SDS-PAGE WB/HPLC detection (Optional) Bradford assay for quantitation Provide COA/report		1 week

Bulk and Custom Product Services

Each project of recombinant protein customization requires evaluation to determine the corresponding plan and price. For further information on service prices or technical details, please send an email to sales@MedChemExpress.com Or call 609-228-6898 to contact us directly.

Salutation:		* Country or Region:
* Applicant Name:		* Organization Name:
* Department:		* Email Address:
* Phone Number
* Product Name:		* Requested quantity：
* Species:		* Source:
*Accession:		* Purity:
* Protein Application：
MCE Japan Authorized Agent:	ナミキ商事株式会社コスモ・バイオ株式会社
Remarks:

Mammalian Expression System

Mammalian cell expression systems can achieve correct protein folding and complex post-translational modifications, making the proteins expressed closer to natural proteins than those from other eukaryotic systems. This has been widely used in vaccine, antibody, and protein development. However, the yield of its transient expression system is relatively low, often requiring scale-up to obtain sufficient proteins, leading to increased cycle times and economic costs. We have a wealth of different series expression and proprietary vectors, which can stably and efficiently express sufficient quantities of recombinant proteins and antibodies.

Advantages of the expression system

01
Can achieve complex post-translational modifications such as glycosylation, resulting in higher biological activity.
02
High protein expression
level, with solid control
elements controlling
protein expression.
03
Transient transfection
allows rapid acquisition
of a certain amount of
secreted soluble protein.

Service Process

	Process	Description	Deliverable	Lead Time
1	Gene Synthesis, Protein Construct Design	Codon optimization; gene synthesis. Subcloning into pTT3, pCEP4, pCMV and pCDNA series expression vectors. Transform plasmids into DH5α or TOP10 competent cells. Plasmid sequencing and preparation.	Purified protein QC COA /Project report	1-2 weeks
2	Protein Expression Evaluation, Optimization	Transient transfection of HEK293F, HEK293T, CHO and other cells. Determine optimal expression conditions.		1-2 weeks
3	Amplified expression and purification	Scale up protein expression using optimized conditions. Multiple-methods purification to obtain sufficient amounts of high-purity soluble proteins.		1-2 weeks
4	QC & Delivery	Purity analysis by SDS-PAGE WB/HPLC detection (Optional) Bradford assay for quantitation Provide COA/report		1 week

Bulk and Custom Product Services

Each project of recombinant protein customization requires evaluation to determine the corresponding plan and price. For further information on service prices or technical details, please send an email to sales@MedChemExpress.com Or call 609-228-6898 to contact us directly.

Salutation:		* Country or Region:
* Applicant Name:		* Organization Name:
* Department:		* Email Address:
* Phone Number
* Product Name:		* Requested quantity：
* Species:		* Source:
*Accession:		* Purity:
* Protein Application：
MCE Japan Authorized Agent:	ナミキ商事株式会社コスモ・バイオ株式会社
Remarks:

Insect Expression System

The Baculovirus-Insect Cell Expression System is one of the eukaryotic expression systems. This system uses baculovirus-infected insect cells for the expression, purification, and large-scale production of recombinant proteins. Due to the large baculovirus genome and multiple restriction enzyme cut sites on its genome, it has strong plasticity in accommodating exogenous genes and advantages in expressing sizeable molecular weight proteins. By modifying the baculovirus to become a highly efficient recombinant viral vector for expressing target proteins, MedChemExpress (MCE) can provide you with expression development for Sf9 and Hi-5 insect cell lines.

Advantages of the expression system

01
High safety: the baculovirus expression vector has high species specificity.
02
Large gene capacity, capable of expressing large molecular weight proteins.
03
Strong operability and high level of exogenous gene expression.
04
Has similar post-translational modifications, such as glycosylation to mammalian cells, resulting in high biological activity.

Service Process

	Process	Description	Deliverable	Lead Time
1	Gene Synthesis, Protein Construct Design	Codon optimization; gene synthesis. Subcloning into an appropriate expression vector, e.g.:pFastBac1, pFastBacHT, etc.. Transform plasmids into DH5α or TOP10 competent cells. Plasmid sequencing and preparation.	Purified protein QC COA /Project report	1-2 weeks
2	Protein Expression Evaluation, Optimization	Transform DH10Bac cells to get recombinant Bacmid. Transfection of insect cells with recombinant Bacmid DNA. Based on protein demand and preliminary expression results, Prepare P1 generation virus (low titer) and P2 generation virus (high titer).		2-3 weeks
3	Amplified expression and purification	Infect insect cells with P1 or P2 generation viruses using optimized conditions. Multiple-methods purification to obtain sufficient amounts of high-purity soluble proteins.		1-2 weeks
4	QC & Delivery	Purity analysis by SDS-PAGE WB/HPLC detection (Optional) Bradford assay for quantitation Provide COA/report		1 week

Bulk and Custom Product Services

Each project of recombinant protein customization requires evaluation to determine the corresponding plan and price. For further information on service prices or technical details, please send an email to sales@MedChemExpress.com Or call 609-228-6898 to contact us directly.

Salutation:		* Country or Region:
* Applicant Name:		* Organization Name:
* Department:		* Email Address:
* Phone Number
* Product Name:		* Requested quantity：
* Species:		* Source:
*Accession:		* Purity:
* Protein Application：
MCE Japan Authorized Agent:	ナミキ商事株式会社コスモ・バイオ株式会社
Remarks:

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