Protein Biology

MCE WB/IP Lysis Buffer is a lysis buffer used to lyse cell or tissue samples under non-denaturing conditions to prepare protein samples. The lysed cell or tissue samples can be used for PAGE, Western Blot, Immunoprecipitation (IP), Co-Immunoprecipitation (Co-IP), ELISA, and other assays.

MCE MCE RIPA Lysis Buffer is one of the most reliable buffers used to lyse cells from both cultured cells and tissues.

MCE NP-40 Lysis Buffer is a relatively mild reliable buffers used to lyse cells from animal, plant tissue and fungi, bacteria etc.

MCE Mammalian Active Protein Extraction Reagent are gentle, non-denaturing detergents designed for the rapid, high-quality, and high-activity extraction of cytoplasmic, nuclear, and membrane proteins from mammalian cells or tissues.

MCE Red Blood Cell Lysis Buffer (10×) primarily contains ammonium chloride and is a ready-to-use solution designed for rapid and effective lysis and removal of anucleated red blood cells from human or mouse blood and tissue samples without affecting white blood cells, normal tissues, or tumor cells.

MCE PBS Powder (1 L of 1×) consists of NaCl, KCl and Phosphate, and is widely used in immunoassay, microbiology and protein experiments.

MCE PBS Powder (2 L of 1×) consists of NaCl, KCl and Phosphate, and is widely used in immunoassay, microbiology and protein experiments.

MCE TBS Powder (1 L of 1×) consists of NaCl, KCl and Tris, and is widely used as washing buffer in immunocytochemistry, FISH or ELISA, and can also be used for antibody dilution and preparation of blocking solution.

MCE Protease Inhibitor Cocktail (EDTA-Free, 100× in DMSO) is a blend of 6 pan-protease inhibitors for protection of protein integrity.

MCE Protease Inhibitor Cocktail, mini-Tablet (EDTA-Free) is a blend of 5 pan-protease inhibitors for protection of protein integrity.

MCE Protease Inhibitor Cocktail, Bacteria (EDTA-Free, 100× in DMSO) protects proteins from degradation by endogenous proteases released during protein extraction and purification.

MCE Protease and Phosphatase Inhibitor Cocktail (EDTA-Free, 10× in ddH₂O) protects protein from degradation by endogenous proteases released during protein extraction and purification.

Phosphatase Inhibitor Cocktail I (100× in DMSO) is a blend of 2 phosphatase inhibitors that can effectively inhibit alkaline phosphatases and serine/threonine phosphatases. It is used to maintain the phosphorylation status of proteins.

Phosphatase Inhibitor Cocktail Ⅱ (100× in ddH₂O) is a blend of 5 phosphatase inhibitors that can broadly inhibit acid phosphatases, alkaline phosphatases, and protein tyrosine phosphatases (PTPs).

Phosphatase Inhibitor Cocktail Ⅲ (100× in DMSO) is a blend of 3 phosphatase inhibitors that can effectively inhibit serine/threonine phosphatases and alkaline phosphatases. It is used to maintain the phosphorylation status of proteins.

MCE Deacetylase Inhibitor Cocktail is a synergistic combination of chemicals designed to preserve the acetylation state of proteins.

MCE Kinase Assay Buffer can be used for assaying kinase protein.

MCE Kinase Inhibitor Cocktail controls the biological activity of kinases. This inhibitor is primarily used for Biochemicals applications.

MCE Bradford Protein Assay Kit is suitable for detecting the total protein content in samples such as serum, plasma, animal tissues, and cells.

MCE Magnetic Stand is optimized for efficient magnetic separation of all types of MCE Magnetic Beads with sample volumes ranging from 10 μL to 15 mL.

MCE Anti-HA Magnetic Beads are used for immunoprecipitation (IP) of specific HA-tagged proteins expressed in bacterial and mammalian cells and in vitro expression systems.

MCE Anti-HA Magnetic Beads (1 μm) are used for immunoprecipitation (IP) of specific HA-tagged proteins expressed in bacterial and mammalian cells andin vitro expression systems.

MCE Protein A/G Magnetic Beads provide a fast and convenient method for Immunoprecipitation, Co-Immunoprecipitation and Chromatin Immunoprecipitation.

Protein A Magnetic Beads provide a fast and convenient method for Immunoprecipitation and Co-Immunoprecipitation and Chromatin Immunoprecipitation.

Protein G Magnetic Beads provide a fast and convenient method for Immunoprecipitation and Co-Immunoprecipitation and Chromatin Immunoprecipitation.

Protein L Magnetic Beads provide a fast and convenient method for Immunuoprecipitaion, Co-Immunoprecipitation and Chromatin Immunoprecipitation.

MCE Anti-c-Myc Magnetic Beads are used for immunoprecipitation (IP) of specific c-Myc-tagged proteins expressed in bacterial and mammalian cells and in vitro expression systems.

MCE Anti-c-Myc Magnetic Beads (1 μm) are used for immunoprecipitation (IP) of specific c-Myc-tagged proteins expressed in bacterial and mammalian cells and in vitro expression systems.

MCE Anti-Flag Magnetic Beads are used for immunoprecipitation (IP) of specific Flag-tagged (DYKDDDDK) proteins expressed in bacterial and mammalian cells and in vitro expression systems, and also suitable for Co-immunoprecipitation and purification of Flag-tagged protein.

MCE Anti-Flag Magnetic Beads are used for immunoprecipitation (IP) of specific Flag-tagged (DYKDDDDK) proteins expressed in bacterial and mammalian cells and in vitro expression systems, and also suitable for Co-immunoprecipitation and purification of Flag-tagged protein.

MCE Streptavidin Magnetic Beads provide a fast and convenient method for numerous applications, including purification of proteins and nucleic acids, protein interaction studies, immunoprecipitation, immunoassays, pull-down and cell isolation.

MCE Anti-His Magnetic Beads are used for immunoprecipitation (IP) of specific His-tagged proteins expressed in bacterial and mammalian cells and in vitro expression systems.

MCE Anti-GST Magnetic Beads are used for immunoprecipitation (IP) of specific GST-tagged proteins expressed in bacterial and mammalian cells and in vitro expression systems.

MCE Hydroxyl Magnetic beads (200 nm, 10 mg/mL) can rapidly isolate nucleic acids from biological samples, which is conducive to the automation and high throughput extraction of nucleic acids.

MCE Amino magnetic beads (200 nm，10 mg/mL) can easily and efficiently combine with a variety of biological ligand in high loads, such as proteins, peptides, oligonucleotides, drug molecules, etc. It can be used as a good basic material for subsequent processing, adsorption, chemical modification and other follow-up processing.

MCE Carboxyl Magnetic beads (200 nm, 10 mg/mL) are characterized by superparamagnetism, fast magnetic response, abundant carboxyl functional groups, monodispersity, and submicron scale particle size. Biological ligands (proteins, peptides, oligonucleotides, drug molecules, etc.) can be covalently coupled to the surface of microspheres under the action of special chemical reagents (such as EDC).

MCE CHO Magnetic Beads (200 nm, 10 mg/mL) contain CHO functional groups, which react with primary amines on proteins or other molecules to form stable amide linkages，can covalently immobilize proteins for the affinity purification of antibodies, antigens and other biomolecules.

MCE NHS Magnetic Beads (200 nm, 10 mg/mL) contain N-hydroxysuccinimide (NHS) functional groups, which react with primary amines on proteins or other molecules to form stable amide linkages，can covalently immobilize proteins for the affinity purification of antibodies, antigens and other biomolecules.

MCE Oligo (dT)₃₀ Magnetic Beads are designed for the rapid isolation of highly purified, intact mRNA from eukaryotic total RNA or directly from crude extracts of cells, plant and animal tissues.

MCE Affinity Chromatography (AC) Columns are designed for purification of recombinant proteins with different tags, enzymes, antibodies, antigens and nucleic acids.

MCE Ni-NTA His-Tag Purification Agarose, a 6% highly cross-linked agarose reagent, enables high-yield, high-purity purification of his-tagged proteins.

MCE Glutathione Agarose, a 4% highly cross-linked agarose reagent, enables high-yield, high-purity purification of GST-tagged proteins.

MCE Protein A Agarose, a 4% highly cross-linked agarose reagent coupled with recombinant Protein A, effectively purifies mammalian monoclonal and polyclonal antibodies, such as human IgG, IgE, IgM.

MCE Protein G Agarose, a 4% highly cross-linked agarose reagent coupled with recombinant Protein G, effectively purifies mammalian monoclonal and polyclonal antibodies, such as human IgG3 and rat IgG2a.

MCE Protein L Agarose, a 4% highly cross-linked agarose reagent coupled with recombinant Protein L, effectively purifies mammalian monoclonal and polyclonal antibodies which have kappa light chain.

MCE Anti-Flag Affinity Gel is used for immunoprecipitation (IP) or protein purification of specific Flag-tagged (DYKDDDDK) proteins expressed in bacterial and mammalian cells and in vitro expression systems.

MCE Streptavidin Agarose 6FF, a 6% highly cross-linked agarose reagent coupled with recombinant streptavidin, is an affinity chromatography medium for separation and purification of biotinylated peptides, antibodies, lectins, etc. The total binding capacity of Streptavidin Agarose 6FF is more than 200 nmol of D-Biotin/mL settled resin.

MCE High-Affinity Iodoacetyl Agarose, a 4% highly cross-linked agarose reagent coupled with a derivative of iodoacetic acid, is ideal for conjugating sulfhydryl-containing peptide or protein for subsequent affinity purification.

MCE Ni-NTA 6FF Prepacked Column is ideal for high performance purification of polyhistidine-tagged proteins expressed in E.coli, yeast, insect and mammalian expression systems.

MCE Anti-GFP Affinity Gel can be used for the detection and purification of GFP, EGFP, their fusion-expressed proteins and IP assays.

MCE Protein A/G Agarose is an affinity chromatography medium for separation and purification of immunoglobulins.

MCE Anti-c-Myc Affinity Gel can be used for the detection and purification of c-Myc fusion expressed proteins, and immunoprecipitation (IP) experiments to detect the expression of recombinant proteins in target cells.

MCE Anti-HA Affinity Gel can be employed for the detection and purification of HA fusion expressed proteins, and can also be used in immunoprecipitation (IP) experiments to detect the expression of recombinant proteins in target cells.

MCE Anti-RFP Affinity Gel can be used for the detection and purification of native RFP, RFP mutants, and IP assays.

MCE Anti-YFP Affinity Gel can be used for the detection and purification of proteins tagged with GFP and EGFP and IP assays.

MCE Ni-IDA Agarose can be used for the detection and purification of His-tag proteins.

MCE Strep-Tag Ⅱ Agarose can be used for the detection and purification of Strep-tag Ⅱ and Twin Strep-tag Ⅱ proteins from various expression sources.

MCE PBS Powder (1 L of 1×) consists of NaCl, KCl and Phosphate, and is widely used in immunoassay, microbiology and protein experiments.

MCE PBS Powder (2 L of 1×) consists of NaCl, KCl and Phosphate, and is widely used in immunoassay, microbiology and protein experiments.

MCE Glutathione Magnetic Agarose Beads have high protein-binding capacity and stability, making it ideal for high performance purification of GST-tagged fusion proteins expressed in E. coli, yeast, insect and mammalian expression systems.

MCE Anti-GFP Magnetic Agarose Beads can be used for the detection and purification of Flag (DYKDDDDK) fusion-expressed proteins and IP assays.

MCE Anti-HA Magnetic Agarose Beads can be used for the detection and purification of HA fusion-expressed proteins and IP assays.

MCE Ni-IDA Magnetic Agarose Beads can be used for the detection and purification of His-tag proteins from various expression sources.

MCE Ni-NTA Magnetic Agarose Beads can be used for the detection and purification of His-tag proteins from various expression sources.

MCE Protein A Plus Magnetic Agarose Beads can be used for the detection and purification of IgG from serum, ascites fluid, cell culture supernatant and other antibody samples.

MCE Protein G Plus Magnetic Agarose Beads can be used for the detection and purification of IgG from serum, ascites fluid, cell culture supernatant and other antibody samples.

MCE Anti-GFP Magnetic Agarose Beads can be used for the detection and purification of GFP, EGFP, and their fusion-expressed proteins and IP assays.

MCE PBS-T Powder (1 L of 1×) consists of NaCl, KCl, Phosphate and Tween 20, and is widely used in ELISA and Western Blot, and can also be used for antibody dilution and preparation of blocking solution.

MCE TBS-T Powder (1 L of 1×) consists of NaCl, KCl, Tris and Tween 20, and is widely used as washing buffer in immunocytochemistry, FISH or ELISA, and can also be used for antibody dilution and preparation of blocking solution.

MCE Tris-MES-SDS Powder (1 L of 1×) consists of Tris, MES, EDTA and SDS, and is mainly used for separation of small- to medium-sized proteins.

MCE Tris-MOPS-SDS Powder (1 L of 1×) consists of Tris, MOPS, EDTA and SDS, and is mainly used for separation of medium- to large-sized proteins.

MCE Tris-Glycine-SDS Powder (1 L of 1×) consists of Tris, Glycine and SDS, and is widely used as an electrophoresis buffer for SDS-PAGE and Western Blot.

MCE SDS dust-free Powder can be directly prepared into 10% SDS solution, and is widely used for protein denaturation.

MCE Tris-HEPES-SDS Powder (500 mL of 1×) consists of Tris, HEPES, EDTA and SDS, and is widely used in protein electrophoresis.

MCE Tris-Glycine Powder (1 L of 1×) consists of Tris and Glycine, and is widely used as an electrophoresis buffer for Native-PAGE.

MCE Transfer Buffer (Semi Dry) Powder (1 L of 1×) consists of Tris, Glycine and SDS, and is suitable for Western Blot semi-dry electrophoresis.

MCE Rapid Blocking Buffer (TBS-T) Powder (100 mL of 1×) mainly consists of fish gelatin protein, and is widely used for the antibody blocking step in Western Blot or ELISA within 15 minutes.

MCE PBS-T Powder (1 L of 1×) consists of NaCl, KCl, Phosphate and Tween 20, and is widely used in ELISA and Western Blot, and can also be used for antibody dilution and preparation of blocking solution.

MCE TBS-T Powder (1 L of 1×) consists of NaCl, KCl, Tris and Tween 20, and is widely used as washing buffer in immunocytochemistry, FISH or ELISA, and can also be used for antibody dilution and preparation of blocking solution.

MCE Ultra High Sensitivity ECL Kit is an ultra-sensitive, luminol-based enhanced chemiluminescent substrate for detecting horseradish peroxidase (HRP) labeled antibodies on western blots.

High Sensitivity ECL Kit enables low picogram detection of antigen by oxidizing luminol in the presence of HRP and peroxide. This reaction produces a prolonged chemiluminescence which can be visualized on X-ray film or digital imaging systems.

Sensitive ECL Kit enables low picogram detection of antigen by oxidizing luminol in the presence of HRP and peroxide. This reaction produces a prolonged chemiluminescence which can be visualized on X-ray film or digital imaging systems.

SDS-PAGE Sample Loading Buffer (4×) is appropriate for loading protein samples on SDS-PAGE.

MCE Coomassie Blue Fast Staining Solution (No Heating, 10×) uses Coomassie Brilliant Blue G250 as the dye. It can be used for rapid, sensitive staining of protein gels in SDS-PAGE or Native-PAGE without contamination, or for detecting residual proteins on PAGE gels after Western Blot transfer.

MCE Antibody Labeling Kit-VF 488 can efficiently and covalently attach the VF 488 dye to antibodies.