Fluorescent labeling Service

Fluorescent labeling technology refers to the covalent binding or physical adsorption of fluorescent substances on a chemical group of molecule to be studied, and the subsequent qualitative, and quantitative analyses of the labeled objects by observing fluorescence characteristics.

Fluorescent labeling has several advantages i.e. it does not cause radioactive contamination, easy to operate, and easy to observe under fluorescent microscope, etc. Fluorescent labelling has great potential in studying proteins, nucleic acids, cell biology and performing immuno-analyses. The application of fluorescent labeling has been deeply involved in many fields such as pharmacology, physiology, environmental science, and information sciences, etc., and has been widely used in the functional research of proteins and drug screening.

MedChemExpress (MCE) has an efficient, experienced and professional technical team who can chemically incorporate fluorescent labels on biomolecules, proteins, antibodies, and peptides. We can offer variety of labels and conjugates to choose from and multiple customization services less to microgram level. Furthermore, we can meet all relevant needs of customers regarding fluorescent labelling.

Advantages

• Professional protein/organic chemistry technical team.

• Professional pre-sale and after-sale technical services.

• High quality label coupling custom services from micrograms to milligrams.

• Services to minimize the steric hindrance and reduce the intervention of marker activity.

Service process

Dye work solution Conjugation reaction Incubate conjugation Purify the conjugation

The process of antibody labeling

Service project	Service requirements	Service period	Service products
Protein labeling	Protein more than 1 mg, purity more than 90%, concentration more than 1 mg/mL	3-10 working days after receiving the sample	Labeled product and Labeling report
Antibody labeling	Antibody more than 100 μg, purity more than 90%, concentration more than 1 mg/mL	3-10 working days after receiving the sample
Peptides labeling	The minimum molecular weight of the peptide is not less than 3 kDa, and the number of amino acids is not less than 30	3-10 working days after receiving the sample
Biomolecule labeling	The minimum starting scalar of biomolecule is not less than 10 mg, the minimum molecular weight is less than 3 kDa	More than 7 working days after receiving the sample, adjust according to sample situation and specific needs

Part of the Dye Information

Cat.No	Product	Optimal Excitation Wavelength(nm)	Optimal Emission Wavelength(nm)	Label Type
HY-66019	FITC	488	525	Amine
HY-D0723	5(6)-TAMRA SE	530	582
HY-D0826	Cy2-SE (iodine)	492	510
HY-112498	Cy3 NHS ester	550	570
HY-D0819A	CY5-SE (triethylamine salt)	650	670
HY-D0824	CY7-SE	750	773
HY-D1085	AMCA-X SE	345	475
HY-N7131	Coumarin 6	554	585
HY-D0042	NBD-Cl	464	512
HY-D0154	TRITC	596	620
HY-131030	Janelia Fluor® 669, SE	669	685
HY-130736	Janelia Fluor® 549, SE	549	635
HY-138658	Janelia Fluor® 526, SE	526	585
HY-B0511	Biotin	/	/
HY-138200	Cy5 maleimide	650	670	Mercapto
HY-D1323	Cyanine5.5 maleimide chloride	678	705
HY-D1566	Sulfo-Cy7.5 maleimide	778	797
HY-D1540	Cyanine5.5 amine	678	705	Carboxyl

Each project needs to be evaluated separately to determine the corresponding lead time and price. For further information on pricing or technical details, please email sales@MedChemExpress.com Or contact MedChemExpress (MCE) sales directly.

* Product Type:		* Product Name:
CAS No.:		* Conjugated:
* Product Quantity:		Conjugated information:
Purity:
Salutation:		* Applicant Name:
* Country or Region:
* Organization Name:		* Email Address:
* Phone number:
MCE Japan Authorized Agent:	ナミキ商事株式会社コスモ・バイオ株式会社
Remarks:

Note:
1. Fluorescent labeling service does not guarantee activity. Antibody labeling services do not affect antibody activity and more than 95% of protein labeling services do not affect protein activity.
2. Conjugation leads to depletion. The recovery of small molecule labeling usually varies from 5% to 60%. For 50-100 μg protein labeling service, recovery is over 90%, for 500 μg or more protein labeling service, recovery is over 98%.
3. There is a risk of failure for small molecule labeling, and a partial fee will be charged if failed.

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