Cell Culture Supplements

L-glutamine is an important amino acid supplement commonly added to mammalian cell culture media. L-glutamine serves as an auxiliary energy source, especially when cells are rapidly dividing. L-glutamine is also important in the production of purine and pyrimidine nucleotides, amino sugars, glutathione, L-glutamate, other amino acids, and plays a role in protein synthesis and glucose production.

MEM Non-essential Amino Acid Solution (100×) has been filtered and can be used directly. It adds nutrients and reduces the biosynthetic burden on cell in vitro.

N-2 Supplement promotes cell growth and survival. It has been associated with the growth and expression of neuroblastoma, and with post-mitotic neuronal survival and expression in primary cultures of both the peripheral nervous system (PNS) and central nervous system (CNS).

MCE N-2 Serum-free Supplement (100×) can be used in conjunction with growth factors such as bFGF and EGF in serum-free neuronal cell culture mediums, DMEM and the DMEM/F12.

The most cited neuronal cell culture supplement, B-27 Supplement is an optimized serum-free supplement used to support the low- or high-density growth and short- or long-term viability of embryonic.

MCE Bi-27 Serum-free Supplement (50×) can be used to support the low- or high-density growth and short- or long-term viability of embryonic.

Bi-27 Additive (50×), Vitamin A-Free is a customized variant of the standard Bi-27 formulation with vitamin A removed as a supplement for neuronal cell culture to support the low- or high-density growth and short- or long-term viability of embryonic.

MCE Bi-27 Serum-free Supplement (50×), Vitamin A-Free can be used to support the low- or high-density growth and short- or long-term viability of embryonic.

MCE HAT Supplement (50×) can be added to cell culture medium as a selection agent for post-fusion selection of HGPRT-myeloma hybridoma cells used for antibody production.

MCE HT Supplement (50×) is a liquid mixture of hypoxanthine (5 mM) and thymidine (0.8 mM), and can be added to cell culture medium as an additive for the DNA salvage synthesis pathway.

MCE ITS Supplement (100×) can be used as a cell culture supplement and added to the medium for serum-free or low-serum cell culture systems.

MCE Lipid Concentrate (Chemically Defined) is a concentrated lipid solution with a clearly defined chemical composition, which can be added to cell culture media as an additive for serum-free or low-serum cell culture systems.