1. MCE Kits
  2. Protein Biology
  3. Protein Sample Preparation

Protein Sample Preparation

FAQ

MCE Kits
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Cell Lysis

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  • HY-K1000 WB/IP Lysis Buffer

    MCE WB/IP Lysis Buffer is a lysis buffer used to lyse cell or tissue samples under non-denaturing conditions to prepare protein samples. The lysed cell or tissue samples can be used for PAGE, Western Blot, Immunoprecipitation (IP), Co-Immunoprecipitation (Co-IP), ELISA, and other assays.

    HY-K1001 RIPA Lysis Buffer (Strong)

    MCE MCE RIPA Lysis Buffer is one of the most reliable buffers used to lyse cells from both cultured cells and tissues.

    HY-K1002 NP-40 Lysis Buffer

    MCE NP-40 Lysis Buffer is a relatively mild reliable buffers used to lyse cells from animal, plant tissue and fungi, bacteria etc.

    HY-K1003 Mammalian Active Protein Extraction Reagent

    MCE Mammalian Active Protein Extraction Reagent are gentle, non-denaturing detergents designed for the rapid, high-quality, and high-activity extraction of cytoplasmic, nuclear, and membrane proteins from mammalian cells or tissues.

    HY-K3010 Red Blood Cell Lysis Buffer

    MCE Red Blood Cell Lysis Buffer (10×) primarily contains ammonium chloride and is a ready-to-use solution designed for rapid and effective lysis and removal of anucleated red blood cells from human or mouse blood and tissue samples without affecting white blood cells, normal tissues, or tumor cells.

  • HY-K1023 PBS Powder (1 L of 1×)

    MCE PBS Powder (1 L of 1×) consists of NaCl, KCl and Phosphate, and is widely used in immunoassay, microbiology and protein experiments.

    HY-K1024 PBS Powder (2 L of 1×)

    MCE PBS Powder (2 L of 1×) consists of NaCl, KCl and Phosphate, and is widely used in immunoassay, microbiology and protein experiments.

    HY-K1026 TBS Powder (1 L of 1×)

    MCE TBS Powder (1 L of 1×) consists of NaCl, KCl and Tris, and is widely used as washing buffer in immunocytochemistry, FISH or ELISA, and can also be used for antibody dilution and preparation of blocking solution.

  • HY-K0021 Phosphatase Inhibitor Cocktail I (100× in DMSO)

    Phosphatase Inhibitor Cocktail I (100× in DMSO) is a blend of 2 phosphatase inhibitors that can effectively inhibit alkaline phosphatases and serine/threonine phosphatases. It is used to maintain the phosphorylation status of proteins.

    HY-K0022 Phosphatase Inhibitor Cocktail Ⅱ (100× in ddH2O)

    Phosphatase Inhibitor Cocktail Ⅱ (100× in ddH2O) is a blend of 5 phosphatase inhibitors that can broadly inhibit acid phosphatases, alkaline phosphatases, and protein tyrosine phosphatases (PTPs).

    HY-K0023 Phosphatase Inhibitor Cocktail Ⅲ (100× in DMSO)

    Phosphatase Inhibitor Cocktail Ⅲ (100× in DMSO) is a blend of 3 phosphatase inhibitors that can effectively inhibit serine/threonine phosphatases and alkaline phosphatases. It is used to maintain the phosphorylation status of proteins.

Deacetylase Inhibitor Cocktail

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Kinase Inhibitor Cocktail

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  • HY-K0016 Kinase Assay Buffer

    MCE Kinase Assay Buffer can be used for assaying kinase protein.

    HY-K0017 Kinase Inhibitor Cocktail

    MCE Kinase Inhibitor Cocktail controls the biological activity of kinases. This inhibitor is primarily used for Biochemicals applications.

Protein Assay

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  • HY-K2001 Bradford Protein Assay Kit

    MCE Bradford Protein Assay Kit is suitable for detecting the total protein content in samples such as serum, plasma, animal tissues, and cells.