Molecular Biology

MCE SYBR Green I Nucleic Acid Gel Stain is one of the most sensitive stains available for detecting double-stranded DNA (dsDNA) in agarose and polyacrylamide gels.

MCE Agarose With TAE Powder (1%) mainly consists of proportionally mixed agarose and TAE Powder, and can be directly made into 1% agarose gel.

MCE Agarose can be made into 0.5-2.5% agarose gel according to different needs and can resolve DNA and RNA fragments from 50-15,000 bp.

MCE TAE Powder (1 L of 1×) consists of Tris-acetate and EDTA-2Na, and is widely used in agarose gel electrophoresis of nucleic acid.

MCE TBE Powder (1 L of 1×) consists of Tris, Boric Acid and EDTA, and is widely used in agarose gel electrophoresis of nucleic acid.

MCE 6× DNA Loading Buffer with SDS is a modified 6-fold buffer and mainly consists of glycerin, EDTA, SDS, Orange G and Xylene Cyanol FF.

The 50 bp DNA Marker is provided in a solution of 1× DNA Loading Buffer, which can be directly used for nucleic acid electrophoresis analysis.

The 100 bp DNA Marker is provided in a solution of 1× DNA Loading Buffer, which can be directly used for nucleic acid electrophoresis analysis.

The 100 bp plus DNA Marker is provided in a solution of 1× DNA Loading Buffer, which can be directly used for nucleic acid electrophoresis analysis.

The 500 bp DNA Marker is provided in a solution of 1× DNA Loading Buffer, which can be directly used for nucleic acid electrophoresis analysis.

The 1 kb DNA Marker is provided in a solution of 1× DNA Loading Buffer, which can be directly used for nucleic acid electrophoresis analysis.

The 2 kb DNA Marker is provided in a solution of 1× DNA Loading Buffer, which can be directly used for nucleic acid electrophoresis analysis.

The 5 kb DNA Marker is provided in a solution of 1× DNA Loading Buffer, which can be directly used for nucleic acid electrophoresis analysis.

The 15 kb DNA Marker is provided in a solution of 1× DNA Loading Buffer, which can be directly used for nucleic acid electrophoresis analysis.

MCE Seamless DNA Assembly Plus Kit contains an optimized mix of recombinase, reaction buffer, and additional cofactors that significantly improve the cloning efficiency and tolerance to impurities. This product can complete multiple DNA fragments recombination and takes only 5 minutes for single fragment, and the positive rate is more than 95%.

MCE Seamless DNA Assembly Ultra Kit is a next-generation recombinant cloning kit that allows the recombination of single or multiple DNA fragments in a single reaction.

MCE T4 DNA Ligase (Fast) is produced by Escherichia coli carrying a T4 phage, catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA.

This enzyme is a DNA ligase cloned from Thermus aquaticus HB8. It is expressed in E. coli using genetic recombination technology and purified and isolated multiple times.

T7 Endonuclease I can recognize and cleave incompletely paired DNA, cruciform structure DNA, Holliday structure or crossover DNA, heterologous double-stranded DNA, or cleave nicked double-stranded DNA at a slower rate.

This product is obtained by expressing the Exo I gene (E. coli) in Escherichia coli and then purifying and isolating it multiple times.

MCE ApaL I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: Alw44 I, Vne I.

MCE Avr II is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: AspA2 I, Bln I, XmaJ I.

MCE Asc I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: PalA I, Sgs I.

MCE BamH I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products.

MCE Bcl I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: Fba I, Ksp22 I.

MCE Bgl II is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products.

MCE Bsa I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: Eco31 I, Bso31 I, BspTN I.

MCE BstB I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: Asu II, Bpu14 I, Bsp119 I, BspT104 I, Nsp V, Sfu I.

MCE BstE II is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: BstP I, Eco91 I, EcoO65 I, PspE I.

MCE Cla I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: BspD I, Bsa29 I, BseC I, Bsu15 I, BsuTU I.

MCE Dpn I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: Mal I.

MCE Dpn II is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: Mbo I, Sau3A I, Bsp143 I, BstMB I, Nde II, BstKT I.

MCE Eag I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: BseX3 I, BstZ I, EclX I, Eco52 I.

MCE EcoR I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: Fun II.

MCE EcoR V is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: Eco32 I.

MCE Esp3 I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: BsmB I.

MCE Fsp I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: Acc16 I，Nsb I.

MCE Hind III is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products.

MCE Hinf I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products.

MCE Hpa I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: KspA I.

MCE Kpn I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: Asp718 I, Acc65 I.

MCE Mlu I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products.

MCE Mnl I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products.

MCE Nco I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: Bsp19 I.

MCE Nde I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: FauND I.

MCE Nhe I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: Bmt I.

MCE Not I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: CciN I.

MCE Nsi I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: EcoT22 I, Mph1103 I, Zsp2 I.

MCE Pac I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products.

MCE Pst I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: BspMA I.

MCE Pvu II is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products.

MCE Sac I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: Psp124B I, Sst I, Ecl136 II, EcoICR I.

MCE Sac II is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: Cfr42 I, Ksp I, Sfr303 I, SgrB I.

MCE Sal I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products.

MCE Sbf I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: Sda I, Sse8387 I.

MCE Sfi I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products.

MCE Sma I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: Cfr9 I, Xma I.

MCE Spe I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: Ahl I, Bcu I.

MCE Sph I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: Pae I.

MCE Ssp I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products.

MCE Stu I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: Eco147 I, Pce I, SseB I.

Taq I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products.

Xba I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products.

Xho I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: PaeR7 I, Sfr274 I, Sla I.

Nru I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: Bsp68 I, BtuM I, Rru I.

Age I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: AsiG I, CspA I, BshT I, PinA I.

BspQ I is one of the endonucleases of Type IIs that recognize non-palindromic sequences and cut outside of the recognition sequence, and is commonly used in Golden Gate assembly. Isoschizomers: Sap I, Lgu I, PciS I.

Sge I restriction enzyme recognizes and cleaves DNA targets containing 5-methylcytosine on one or both DNA strands, and cuts best at 37℃.

BsmB I is one of the endonucleases of Type IIs that recognize non-palindromic sequences and cut outside of the recognition sequence, and is commonly used in Golden Gate assembly. Isoschizomers: Esp3 I, BstGZ53 I, Esp16 I, Esp23 I.

Bsa I, ADCF is animal derived component free, and has good redundancy of enzyme activity, which can easily cope with excessive substrate or difficult template enzyme cutting.

BsmB I, ADCF is animal derived component free, and has good redundancy of enzyme activity, which can easily cope with excessive substrate or difficult template enzyme cutting. Isoschizomers: Esp3 I, BstGZ53 I, Esp16 I, Esp23 I.

EcoR I, ADCF is animal derived component free, and has good redundancy of enzyme activity, which can easily cope with excessive substrate or difficult template enzyme cutting. Isoschizomers: Fun II.

Xba I, ADCF is animal derived component free, and has good redundancy of enzyme activity, which can easily cope with excessive substrate or difficult template enzyme cutting.

RNase R is a magnesium ion-dependent 3'-5' exoribonuclease that can increase the abundance of circular RNA by degrading linear RNA in mixed RNA, thereby enriching circular RNA.

RNase III is a specific exonuclease expressed in E.coli that can cleave dsRNA into 12-15 bp dsRNA fragments.

O-Glycosidase is highly specific and can release Galβ1-3GalNAc from serine, threonine residues or as part of a glycopeptide or glycoprotein. Applied to glycoprotein biosynthesis analysis, O-glycan bioactive protein O-glycosylation detection and binding site analysis.

RNase H is an endoribonuclease expressed and purified from Escherichia coli and can specifically hydrolyze RNA in DNA-RNA hybrid strands.

FnCas12a(Cpf1), is an RNA-guided, DNA-editable recombinant endonuclease that can be used for gene editing and detection.

Lambda DNA (λ DNA) is the DNA in λ bacteriophage, which is used as the substrate of restriction endonuclease. This product is methylated Lambda DNA.

96/384-well Storage Plate Lid can be used for dust prevention for 96/384-well Storage Plate.

The MedChemExpress^® 8-Channel Handheld Screw Cap Decapper is a necessary tool for handling thousands of screw cap tubes. It can cap and open various types of screw cap tubes, making it convenient and fast to operate. This improves experimental efficiency and meets different needs.

The MedChemExpress^® Round Cap Drivers for 8-Channel Handheld Screw Cap Decapper is compatible with 8-Channel Handheld Screw Cap Decapper (HY-E0156), which can be used for Capping/Recapping FluidX Screw Caps.

MCE 2× High-Fidelity PCR Master Mix (with Dye) is a new type of ready-to-use high-fidelity PCR amplification master mix.During the reaction, only template and primers need to be added to quickly and efficiently complete the PCR process, reducing the risk of contamination during operation.

MCE qPCR Master Mix is provided as a simple-to-use, stabilized 2× formulation that includes all components for qPCR except sample DNA, primers and water.

MCE 2-Color Tracking SYBR qPCR Master Mix (Universal) is a 2× concentration premixed reagent utilizing a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR Green I based quantitative PCR (qPCR).

Phi29 DNA Polymerase is a mesophilic DNA polymerase cloned from Bacillus subtilis phage Phi29.

Bst DNA Polymerase is derived from Bacillus stearothermophilus and has 5'→3' DNA polymerase activity and double-stranded specific 5'→3' exonuclease activity.

Bst DNA Polymerase large fragment is a part of Bacillus stearothermophilus DNA polymerase, which is derived from E. coli strain. It is expressed in E. coli and purified and isolated multiple times.

This product is a DNA polymerase obtained by expressing DNA Polymerase I (E. coli) in E. coli and purifying and isolating it multiple times.

T4 DNA polymerase has 5´→3´ DNA polymerase activity and can catalyze the synthesis of DNA along the 5´→3´ direction with the presence of templates and primers.

Phi29 DNA polymerase is a protein-engineered Phi29 DNA polymerase expressed in E. coli and then purified and isolated multiple times. Compared with wild-type Phi29 DNA polymerase, it has higher amplification efficiency and sensitivity, and can greatly shorten the reaction time.

MCE RT Master Mix for qPCR II is a convenient, ready-to-use formulation for reverse transcription. With advanced reverse transcriptase, this kit can synthesize cDNA more rapidly, more specifically.

MCE RT Master Mix for qPCR (gDNA digester plus) is a convenient, ready-to-use formulation for reverse transcription and can eliminate genomic DNA (gDNA) contaminations in RNA samples. The cDNA product can be directly applied as a template in a standard PCR and real time quantitative PCR (qPCR).

MCE RT Master Mix for qPCR II is a convenient, ready-to-use formulation for reverse transcription and can eliminate genomic DNA (gDNA) contaminations in RNA samples. With updated reverse transcriptase, this kit can synthesize cDNA more rapidly, more specifically.

MCE microRNA RT Master Mix for qPCR Ⅲ (poly A) uses poly(A)-tailing method to perform reverse transcription from miRNA first-strand to cDNA.

Reverse Transcriptase is a reverse transcriptase that clones and expresses the deletion mutant RNase H- of M-MuLV through genetic recombination technology.

RNase Inhibitor is a 50 kDa recombinant protein expressed in E.coli, and can specifically inhibit RNase A, B and C.

Terminal Deoxynucleotidyl Transferase (TdT) is a template-independent DNA polymerase that catalyzes the binding of deoxynucleotides to the 3' hydroxyl end of oligonucleotides, single-stranded DNA, or double-stranded DNA.