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  3. Protein Purification

Protein Purification

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MCE Kits
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Magnetic Stand

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  • HY-K0200 Magnetic Stand

    MCE Magnetic Stand is optimized for efficient magnetic separation of all types of MCE Magnetic Beads with sample volumes ranging from 10 μL to 15 mL.

  • HY-K0201 Anti-HA Magnetic Beads

    MCE Anti-HA Magnetic Beads are used for immunoprecipitation (IP) of specific HA-tagged proteins expressed in bacterial and mammalian cells and in vitro expression systems.

    HY-K0201A Anti-HA Magnetic Beads (1 μm)

    MCE Anti-HA Magnetic Beads (1 μm) are used for immunoprecipitation (IP) of specific HA-tagged proteins expressed in bacterial and mammalian cells andin vitro expression systems.

    HY-K0202 Protein A/G Magnetic Beads

    MCE Protein A/G Magnetic Beads provide a fast and convenient method for Immunoprecipitation, Co-Immunoprecipitation and Chromatin Immunoprecipitation.

    HY-K0203 Protein A Magnetic Beads

    Protein A Magnetic Beads provide a fast and convenient method for Immunoprecipitation and Co-Immunoprecipitation and Chromatin Immunoprecipitation.

    HY-K0204 Protein G Magnetic Beads

    Protein G Magnetic Beads provide a fast and convenient method for Immunoprecipitation and Co-Immunoprecipitation and Chromatin Immunoprecipitation.

    HY-K0205 Protein L Magnetic Beads

    Protein L Magnetic Beads provide a fast and convenient method for Immunuoprecipitaion, Co-Immunoprecipitation and Chromatin Immunoprecipitation.

    HY-K0206 Anti-c-Myc Magnetic Beads

    MCE Anti-c-Myc Magnetic Beads are used for immunoprecipitation (IP) of specific c-Myc-tagged proteins expressed in bacterial and mammalian cells and in vitro expression systems.

    HY-K0206A Anti-c-Myc Magnetic Beads (1 μm)

    MCE Anti-c-Myc Magnetic Beads (1 μm) are used for immunoprecipitation (IP) of specific c-Myc-tagged proteins expressed in bacterial and mammalian cells and in vitro expression systems.

    HY-K0207 Anti-Flag Magnetic Beads

    MCE Anti-Flag Magnetic Beads are used for immunoprecipitation (IP) of specific Flag-tagged (DYKDDDDK) proteins expressed in bacterial and mammalian cells and in vitro expression systems, and also suitable for Co-immunoprecipitation and purification of Flag-tagged protein.

    HY-K0207A Anti-Flag Magnetic Beads (1 μm)

    MCE Anti-Flag Magnetic Beads are used for immunoprecipitation (IP) of specific Flag-tagged (DYKDDDDK) proteins expressed in bacterial and mammalian cells and in vitro expression systems, and also suitable for Co-immunoprecipitation and purification of Flag-tagged protein.

    HY-K0208 Streptavidin Magnetic Beads

    MCE Streptavidin Magnetic Beads provide a fast and convenient method for numerous applications, including purification of proteins and nucleic acids, protein interaction studies, immunoprecipitation, immunoassays, pull-down and cell isolation.

    HY-K0209 Anti-His Magnetic Beads

    MCE Anti-His Magnetic Beads are used for immunoprecipitation (IP) of specific His-tagged proteins expressed in bacterial and mammalian cells and in vitro expression systems.

    HY-K0222 Anti-GST Magnetic Beads

    MCE Anti-GST Magnetic Beads are used for immunoprecipitation (IP) of specific GST-tagged proteins expressed in bacterial and mammalian cells and in vitro expression systems.

    HY-K0223 Hydroxyl Magnetic Beads (200 nm, 10 mg/mL)

    MCE Hydroxyl Magnetic beads (200 nm, 10 mg/mL) can rapidly isolate nucleic acids from biological samples, which is conducive to the automation and high throughput extraction of nucleic acids.

    HY-K0224 Amino Magnetic Beads (200 nm, 10 mg/mL)

    MCE Amino magnetic beads (200 nm,10 mg/mL) can easily and efficiently combine with a variety of biological ligand in high loads, such as proteins, peptides, oligonucleotides, drug molecules, etc. It can be used as a good basic material for subsequent processing, adsorption, chemical modification and other follow-up processing.

    HY-K0225 Carboxyl Magnetic Beads (200 nm, 10 mg/mL)

    MCE Carboxyl Magnetic beads (200 nm, 10 mg/mL) are characterized by superparamagnetism, fast magnetic response, abundant carboxyl functional groups, monodispersity, and submicron scale particle size. Biological ligands (proteins, peptides, oligonucleotides, drug molecules, etc.) can be covalently coupled to the surface of microspheres under the action of special chemical reagents (such as EDC).

    HY-K0226 CHO Magnetic Beads (200 nm, 10 mg/mL)

    MCE CHO Magnetic Beads (200 nm, 10 mg/mL) contain CHO functional groups, which react with primary amines on proteins or other molecules to form stable amide linkages,can covalently immobilize proteins for the affinity purification of antibodies, antigens and other biomolecules.

    HY-K0227 NHS Magnetic Beads (200nm, 10 mg/mL)

    MCE NHS Magnetic Beads (200 nm, 10 mg/mL) contain N-hydroxysuccinimide (NHS) functional groups, which react with primary amines on proteins or other molecules to form stable amide linkages,can covalently immobilize proteins for the affinity purification of antibodies, antigens and other biomolecules.

    HY-K0228 Oligo (dT)30 Magnetic Beads

    MCE Oligo (dT)30 Magnetic Beads are designed for the rapid isolation of highly purified, intact mRNA from eukaryotic total RNA or directly from crude extracts of cells, plant and animal tissues.

Affinity Chromatography Column

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  • HY-K0221 Affinity Chromatography Column

    MCE Affinity Chromatography (AC) Columns are designed for purification of recombinant proteins with different tags, enzymes, antibodies, antigens and nucleic acids.

  • HY-K0210 Ni-NTA His-Tag Purification Agarose

    MCE Ni-NTA His-Tag Purification Agarose, a 6% highly cross-linked agarose reagent, enables high-yield, high-purity purification of his-tagged proteins.

    HY-K0211 Glutathione Agarose

    MCE Glutathione Agarose, a 4% highly cross-linked agarose reagent, enables high-yield, high-purity purification of GST-tagged proteins.

    HY-K0213 Protein A Agarose

    MCE Protein A Agarose, a 4% highly cross-linked agarose reagent coupled with recombinant Protein A, effectively purifies mammalian monoclonal and polyclonal antibodies, such as human IgG, IgE, IgM.

    HY-K0214 Protein G Agarose

    MCE Protein G Agarose, a 4% highly cross-linked agarose reagent coupled with recombinant Protein G, effectively purifies mammalian monoclonal and polyclonal antibodies, such as human IgG3 and rat IgG2a.

    HY-K0215 Protein L Agarose

    MCE Protein L Agarose, a 4% highly cross-linked agarose reagent coupled with recombinant Protein L, effectively purifies mammalian monoclonal and polyclonal antibodies which have kappa light chain.

    HY-K0217 Anti-Flag Affinity Gel

    MCE Anti-Flag Affinity Gel is used for immunoprecipitation (IP) or protein purification of specific Flag-tagged (DYKDDDDK) proteins expressed in bacterial and mammalian cells and in vitro expression systems.

    HY-K0218A Streptavidin Agarose 6FF

    MCE Streptavidin Agarose 6FF, a 6% highly cross-linked agarose reagent coupled with recombinant streptavidin, is an affinity chromatography medium for separation and purification of biotinylated peptides, antibodies, lectins, etc. The total binding capacity of Streptavidin Agarose 6FF is more than 200 nmol of D-Biotin/mL settled resin.

    HY-K0219 High-Affinity Iodoacetyl Agarose

    MCE High-Affinity Iodoacetyl Agarose, a 4% highly cross-linked agarose reagent coupled with a derivative of iodoacetic acid, is ideal for conjugating sulfhydryl-containing peptide or protein for subsequent affinity purification.

    HY-K0220 Ni-NTA 6FF Prepacked Column

    MCE Ni-NTA 6FF Prepacked Column is ideal for high performance purification of polyhistidine-tagged proteins expressed in E.coli, yeast, insect and mammalian expression systems.

    HY-K0229 Anti-GFP Affinity Gel

    MCE Anti-GFP Affinity Gel can be used for the detection and purification of GFP, EGFP, their fusion-expressed proteins and IP assays.

    HY-K0230 Protein A/G Agarose

    MCE Protein A/G Agarose is an affinity chromatography medium for separation and purification of immunoglobulins.

    HY-K0231 Anti-c-Myc Affinity Gel

    MCE Anti-c-Myc Affinity Gel can be used for the detection and purification of c-Myc fusion expressed proteins, and immunoprecipitation (IP) experiments to detect the expression of recombinant proteins in target cells.

    HY-K0232 Anti-HA Affinity Gel

    MCE Anti-HA Affinity Gel can be employed for the detection and purification of HA fusion expressed proteins, and can also be used in immunoprecipitation (IP) experiments to detect the expression of recombinant proteins in target cells.

    HY-K0233 Anti-RFP Affinity Gel

    MCE Anti-RFP Affinity Gel can be used for the detection and purification of native RFP, RFP mutants, and IP assays.

    HY-K0235 Anti-YFP Affinity Gel

    MCE Anti-YFP Affinity Gel can be used for the detection and purification of proteins tagged with GFP and EGFP and IP assays.

    HY-K0238 Ni-IDA Agarose

    MCE Ni-IDA Agarose can be used for the detection and purification of His-tag proteins.

    HY-K0239 Strep-Tag Ⅱ Agarose

    MCE Strep-Tag Ⅱ Agarose can be used for the detection and purification of Strep-tag Ⅱ and Twin Strep-tag Ⅱ proteins from various expression sources.

  • HY-K1023 PBS Powder (1 L of 1×)

    MCE PBS Powder (1 L of 1×) consists of NaCl, KCl and Phosphate, and is widely used in immunoassay, microbiology and protein experiments.

    HY-K1024 PBS Powder (2 L of 1×)

    MCE PBS Powder (2 L of 1×) consists of NaCl, KCl and Phosphate, and is widely used in immunoassay, microbiology and protein experiments.

  • HY-K0234 Glutathione Magnetic Agarose Beads

    MCE Glutathione Magnetic Agarose Beads have high protein-binding capacity and stability, making it ideal for high performance purification of GST-tagged fusion proteins expressed in E. coli, yeast, insect and mammalian expression systems.

    HY-K0236 Anti-Flag Magnetic Agarose Beads

    MCE Anti-GFP Magnetic Agarose Beads can be used for the detection and purification of Flag (DYKDDDDK) fusion-expressed proteins and IP assays.

    HY-K0237 Anti-HA Magnetic Agarose Beads

    MCE Anti-HA Magnetic Agarose Beads can be used for the detection and purification of HA fusion-expressed proteins and IP assays.

    HY-K0240 Ni-IDA Magnetic Agarose Beads

    MCE Ni-IDA Magnetic Agarose Beads can be used for the detection and purification of His-tag proteins from various expression sources.

    HY-K0241 Ni-NTA Magnetic Agarose Beads

    MCE Ni-NTA Magnetic Agarose Beads can be used for the detection and purification of His-tag proteins from various expression sources.

    HY-K0242 Protein A Plus Magnetic Agarose Beads

    MCE Protein A Plus Magnetic Agarose Beads can be used for the detection and purification of IgG from serum, ascites fluid, cell culture supernatant and other antibody samples.

    HY-K0243 Protein G Plus Magnetic Agarose Beads

    MCE Protein G Plus Magnetic Agarose Beads can be used for the detection and purification of IgG from serum, ascites fluid, cell culture supernatant and other antibody samples.

    HY-K0246 Anti-GFP Magnetic Agarose Beads

    MCE Anti-GFP Magnetic Agarose Beads can be used for the detection and purification of GFP, EGFP, and their fusion-expressed proteins and IP assays.

  • HY-K1022 PBS-T Powder (1 L of 1×)

    MCE PBS-T Powder (1 L of 1×) consists of NaCl, KCl, Phosphate and Tween 20, and is widely used in ELISA and Western Blot, and can also be used for antibody dilution and preparation of blocking solution.

    HY-K1025 TBS-T Powder (1 L of 1×)

    MCE TBS-T Powder (1 L of 1×) consists of NaCl, KCl, Tris and Tween 20, and is widely used as washing buffer in immunocytochemistry, FISH or ELISA, and can also be used for antibody dilution and preparation of blocking solution.