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  3. Janelia Fluor® 585, SE

Janelia Fluor® 585, SE  (Synonyms: JF585, SE; JF585, NHS)

Cat. No.: HY-131025
Handling Instructions

Janelia Fluor 585, SE (JF585, SE) is an orange fluorescent dye containing an NHS ester that can be conjugated with primary amine groups. Janelia Fluor 585, SE can be used immediately for structured illumination (SIM) and stimulated emission depletion (STED) imaging and could be converted to photoactivatable derivative for single-molecule localization microscopy (SMLM) experiments. Janelia Fluor products are licensed under U.S. Pat. Nos. 9,933,417, 10,018,624 and 10,161,932 and other patents from Howard Hughes Medical Institute.

For research use only. We do not sell to patients.

Janelia Fluor® 585, SE Chemical Structure

Janelia Fluor® 585, SE Chemical Structure

CAS No. : 1811539-88-4

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Description

Janelia Fluor 585, SE (JF585, SE) is an orange fluorescent dye containing an NHS ester that can be conjugated with primary amine groups. Janelia Fluor 585, SE can be used immediately for structured illumination (SIM) and stimulated emission depletion (STED) imaging and could be converted to photoactivatable derivative for single-molecule localization microscopy (SMLM) experiments[1]. Janelia Fluor products are licensed under U.S. Pat. Nos. 9,933,417, 10,018,624 and 10,161,932 and other patents from Howard Hughes Medical Institute.

In Vitro

Maximum absorption wavelength (λabs)=585 nm, maximum emission wavelength (λem)=609 nm[1].
The JF585-SNAP-tag ligand (24) also functioned as a cellular label. The new HaloTag ligands derived from JF585 and JF635 show a high degree of chromogenicity and fluorogenicity, a critical parameter in advanced imaging experiments[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

649.59

Formula

C34H27F4N3O6

CAS No.
Emission (Em)

585

Excitation (Ex)

565

SMILES

FC(C/1)(F)C[N+]1=C2C=C3C(C)(C)C4=C(C=CC(N5CC(F)(F)C5)=C4)C(C6=CC(C(ON7C(CCC7=O)=O)=O)=CC=C6C([O-])=O)=C3C=C/2

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation
References
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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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