2. Fluorescent Dye
  3. Biological Conjugation

Biological Conjugation

 

Biological Conjugation (274):

Cat. No. Product Name CAS No. Purity Chemical Structure
  • HY-66019
    FITC 3326-32-7 98.60%
    FITC (Fluorescein Isothiocyanate), is one of the green fluorescein derivatives widely used in biology. FITC has the characteristics of high absorptivity and excellent fluorescence quantum yield. The isothiocyanate group of FITC can be combined with amino, sulfhydryl, imidazole, tyrosyl, carbonyl and other groups on the protein, so as to achieve protein labeling including antibodies and lectins. In addition to its use as a protein marker, FITC can also be used as a fluorescent protein tracer to rapidly identify pathogens by labeling antibodies, or for microsequencing of proteins and peptides (HPLC). The maximum excitation wavelength of FITC is 494 nm. Once excited, it fluoresces yellow-green at a maximum emission wavelength of 520 nm. In addition, FITC is also a hapten that can induce contact hypersensitivity (CHS) and induce an atopic dermatitis model.
    FITC
  • HY-D0924
    Cy5.5 210892-23-2 99.88%
    Cy5.5 is a CY dye. CY, short for Cyanine, is a compound consisting of two nitrogen atoms connected by an odd number of methyl units. Cyanine compounds have the characteristics of long wavelength, adjustable absorption and emission, high extinction coefficient, good water solubility and relatively simple synthesis. CY dyes are of en used for the labeling of proteins, antibodies and small molecular compounds. For the labeling of protein antibodies, the combination can be completed through a simple mixing reaction. Below, we introduce the labeling method of protein antibody labeling, which has certain reference significance.
    Cy5.5
  • HY-D0821
    CY5 146368-11-8 99.66%
    CY5 is a CY dye. CY, short for Cyanine, is a compound consisting of two nitrogen atoms connected by an odd number of methyl units. Cyanine compounds have the characteristics of long wavelength, adjustable absorption and emission, high extinction coefficient, good water solubility and relatively simple synthesis. CY dyes are of en used for the labeling of proteins, antibodies and small molecular compounds. For the labeling of protein antibodies, the combination can be completed through a simple mixing reaction. Below, we introduce the labeling method of protein antibody labeling, which has certain reference significance.
    CY5
  • HY-D0819
    CY5-SE 146368-14-1 98.10%
    Cy5-SE (Cy5 NHS Ester) is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides. This dye requires small amount of organic co-solvent (such as DMF or DMSO) to be used in labeling reaction. This reagent is ideal for very cost-efficient labeling of soluble proteins, as well as all kinds of peptides and oligonucleotides. This reagent also works well in organic solvents for small molecule labeling. Excitation (nm):649, Emission (nm): 670.
    CY5-SE
  • HY-143218
    TPE-MI 1245606-71-6 98.54%
    TPE-MI (Tetraphenylethene maleimide) is a thiol probe for measuring unfolded protein load and proteostasis in cells. TPE-MI can report imbalances in proteostasis in induced pluripotent stem cell models of Huntington disease, as well as cells transfected with mutant Huntington exon 1 before the formation of visible aggregates. TPE-MI also detects protein damage following dihydroartemisinin research of the malaria parasitesPlasmodium falciparum .
    TPE-MI
  • HY-121986
    Yellow AB 85-84-7
    Yellow AB (Solvent yellow 5) is an azo dye that can be used as food dye.
    Yellow AB
  • HY-D2395
    B-Phycoerythrin
    B-Phycoerythrin (B-PE) is a light-harvesting pigment protein that can be isolated from the red alga Porphyridium cruentum. The absorption spectrum of B-Phycoerythrin peaks at 545 nm and 563 nm, with a shoulder at 498 nm.
    B-Phycoerythrin
  • HY-D2397
    Cross-Linked Allophycocyanin
    Cross-Linked Allophycocyanin is a trimer biliprotein with maximum absorption at 650 nm.
    Cross-Linked Allophycocyanin
  • HY-D1614
    BODIPY 493/503 methyl bromide 216434-81-0 ≥98.0%
    BODIPY493/503 methyl bromide is a BODIPY dye. BODIPY dye is a small molecule dye with strong ultraviolet absorption ability, its fluorescence peak is relatively sharp, and the quantum yield is high. They are relatively insensitive to the polarity and pH of the environment and are relatively stable under different physiological conditions. Due to its structural asymmetry, BODIPY derives a variety of structural products. BODIPY lipid droplet dyes can well pass through the cell membrane into the cell, and localize the neutral lipids in the cell to specifically stain the lipid droplets, which can be used for labeling of live cells and fixed cells. Maximum excitation/emission wavelength: 493/503 nm.
    BODIPY 493/503 methyl bromide
  • HY-D0822
    CY3 146368-13-0 99.87%
    Cy3 (Sulfo-Cyanine3) is an orange-fluorescent label for protein and nucleic acid (λex=554, λem=568).
    CY3
  • HY-100041
    Bromobimane 71418-44-5 99.31%
    Bromobimane (Monobromobimane) is a nonfluorescent and converts into fluorescent products when reacts with thiols. Bromobimane has potential applications in labeling thiols.
    Bromobimane
  • HY-D0098
    Fluorescein-5-maleimide 75350-46-8 99.05%
    Fluorescein-5-maleimide (N-(5-Fluoresceinyl)maleimide) is a fluorescent dye. Fluorescein-5-maleimide can be used to detect the redox state of thiols in eukaryotic cells. Fluorescein-5-maleimide can label peptides and is used to detect negatively charged nanoparticles. Fluorescein-5-maleimide can also label actin to explore its interaction with cardiac myosin-binding protein C (cMyBP-C), which helps in developing small molecule modulators for heart failure. Fluorescein-5-maleimide can screen mutant proteins that contain cysteine residues. The excitation wavelength of Fluorescein-5-maleimide is 494 nm, and the emission wavelength is 519 nm.
    Fluorescein-5-maleimide
  • HY-D1583
    Cyanine5 DBCO 2360411-64-7 99.65%
    Cyanine5 DBCO (DBCO-Cy5) is a low-toxicity azide reactive probe (NIR fluorescent dye), for imaging azide-labeled biomolecules via a copper-free "click-through" reaction. Cyanine5 DBCO has no apparent cytotoxicity or animal toxicity and shows no damage to the physiological functions of cells other than the target cells (azide-labeled cells). Cyanine5 DBCO can be used to label and track cells in vitro and in vivo (Ex=635 nm, Em=650-700 nm).
    Cyanine5 DBCO
  • HY-112498
    Cy3 NHS ester 1393363-07-9 99.45%
    Cy3 NHS ester is a CY dye. CY, short for Cyanine, is a compound consisting of two nitrogen atoms connected by an odd number of methyl units. Cyanine compounds have the characteristics of long wavelength, adjustable absorption and emission, high extinction coefficient, good water solubility and relatively simple synthesis. CY dyes are of en used for the labeling of proteins, antibodies and small molecular compounds. For the labeling of protein antibodies, the combination can be completed through a simple mixing reaction. Below, we introduce the labeling method of protein antibody labeling, which has certain reference significance.
    Cy3 NHS ester
  • HY-D1540
    Cyanine5.5 amine 2097714-45-7 99.28%
    Cyanine5.5 amine (Cy 5.5 amine), a Cy5.5 Analogue, is a near-infrared (NIR) fluorescent dye (Ex=648 nm, Em=710 nm). Cyanine5.5 amine can be used in the preparation of Cy5.5-labeled nanoparticles, which can be tracked and imaged with low fluorescence background using confocal microscopy.
    Cyanine5.5 amine
  • HY-15942
    5-TAMRA 91809-66-4 99.25%
    5-TAMRA can produce bright, pH-insensitive orange-red fluorescence (excitation and emission extremes of 546/579) and has good photostability.
    5-TAMRA
  • HY-D1069
    DBCO-Cy3 1782950-79-1 98.00%
    DBCO-Cy3 (DBCO-Sulfo-Cy3) is the derivative of Cyanine3 fluorophore, a pH insensitive from pH (4-10) orange fluorescent dyewith excitation maximum 555 nm and emission maximum of 580nm. DBCO-Cy3 has fast reaction kinetics and good stability, and is productive to use in many standard fluorescent instrumentations. DBCO-Cy3 is a click chemistry reagent, it contains a DBCO group that can undergo strain-promoted alkyne-azide cycloaddition (SPAAC) with molecules containing Azide groups.
    DBCO-Cy3
  • HY-D0832
    CY5-N3 1621101-43-6 99.71%
    CY5-N3 (Sulfo-Cyanine5-azide) is a Cy5-azide, which is a fluorescent dye. CY5-N3 can be used in cell imagine by Click reaction. It contains an azide group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing alkyne groups. It can also undergo ring strain-promoted alkyne-azide cycloaddition (SPAAC) with molecules containing DBCO or BCN groups.
    CY5-N3
  • HY-D0722
    5(6)-CFDA 124387-19-5 99.31%
    5(6)-CFDA is a common aliphatic luciferin-line organism. CFDA conducts free diffusion into cells, and then it is hydrolyzed into carboxyl fluorescein (CF) by intracellular non-specific lipase. CF containing portion contains an additional negative charge so that it is better retained in cells, compared to fluorescein dyes.
    5(6)-CFDA
  • HY-D1808
    Vari Fluor 488-Streptavidin
    Vari Fluor 488-Streptavidin is a dye marker of Vari Fluor-streptavidin consisting of labeling streptavidin with a Vari Fluor series of fluorescent probes. Streptavidin is a high-affinity tetramer protein, each tetramer consisting of four identical streptavidin subunits. Streptavidin binds to biotin specifically via a reversible non-covalent effect. Streptavidin can achieve rapid and efficient detection of biotin markers, and is often used in immunofluorescence (IF), enzyme-linked immunosorbent assay (ELISA), immunohistochemical staining (IFH), in situ hybridization (ISH) and other experiments. Ex/Em=490 nm/515 nm.
    Vari Fluor 488-Streptavidin