HIPK2 Protein, Human (Sf9, GST)

HIPK2 is a multifunctional serine/threonine protein kinase that regulates transcription, p53-mediated apoptosis, and the cell cycle. As a corepressor of factors such as SMAD1 and POU4F1, HIPK2 phosphorylates a variety of substrates, including PDX1, ATF1, PML, p53, CREB1, CTBP1, CBX4, RUNX1, EP300, CTNNB1, HMGA1, ZBTB4, and DAZAP2. HIPK2 Protein, Human (Sf9, GST) is the recombinant human-derived HIPK2 protein, expressed by Sf9 insect cells , with N-GST labeled tag. The total length of HIPK2 Protein, Human (Sf9, GST) is 1197 a.a., .

HIPK2, a serine/threonine-protein kinase, emerges as a versatile regulator, participating in transcriptional control, p53/TP53-mediated cellular apoptosis, and cell cycle modulation. Functioning as a corepressor for various transcription factors, including SMAD1, POU4F1/Brn3a, and potentially NK homeodomain transcription factors, HIPK2 exerts its influence through the phosphorylation of a spectrum of substrates, including PDX1, ATF1, PML, p53/TP53, CREB1, CTBP1, CBX4, RUNX1, EP300, CTNNB1, HMGA1, ZBTB4, and DAZAP2. HIPK2 acts as a negative regulator through the phosphorylation-induced proteasomal degradation of CTNNB1 and the antiapoptotic factor CTBP1. Additionally, it serves as an intermediate kinase in the Wnt/beta-catenin signaling pathway, promoting the proteasomal degradation of MYB. HIPK2's intricate involvement in cellular responses includes transcriptional co-suppression of HIF1A in response to hypoxia and the activation of TP73. Furthermore, it plays a crucial role in DNA damage responses, stabilizing PML, triggering ZBTB4 protein degradation, and preventing DAZAP2-dependent ubiquitination of HIPK2. Moreover, HIPK2 contributes to angiogenesis, erythroid differentiation, and the regulation of eye development during late embryogenesis.

Human

Sf9 insect cells

N-GST

Q9H2X6 (A2-I1198)

28996

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.