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  4. Boc-Val-Pro-Arg-AMC hydrochloride

Boc-Val-Pro-Arg-AMC hydrochloride 

Cat. No.: HY-137784 Purity: 99.31%
COA Handling Instructions

Molarity Calculator

Boc-Val-Pro-Arg-AMC hydrochloride is a sensitive fluorogenic substrate for measuring trypsin-like serine proteases activity.

For research use only. We do not sell to patients.

Boc-Val-Pro-Arg-AMC hydrochloride Chemical Structure

Boc-Val-Pro-Arg-AMC hydrochloride Chemical Structure

CAS No. : 70375-24-5

Size Price Stock Quantity
1 mg USD 60 In-stock
5 mg USD 150 In-stock
10 mg USD 250 In-stock
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100 mg   Get quote  

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Based on 1 publication(s) in Google Scholar

Other Forms of Boc-Val-Pro-Arg-AMC hydrochloride:

Boc-Val-Pro-Arg-AMC hydrochloride Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

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Description

Boc-Val-Pro-Arg-AMC hydrochloride is a sensitive fluorogenic substrate for measuring trypsin-like serine proteases activity[1].

In Vitro

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Trypsin activity assay[2]
1. Add diluted Trypsin enzyme (100 μL) to 800 μL of 50 mM Tris–HCl buffer (pH 8.0).
2. The reaction is immediately initiated by the addition of 100 μL of 50 μM substrate Boc-Val-Pro-Arg-AMC.
3. Incubate at 55 °C for 10 min.
4. Add 1.5 mL of the stopping agent (methyl alcohol:n-butyl alcohol:distilled water = 35:30:35, v/v/v) to stop the reaction.
5. Measure the fluorescence intensity by a spectrofluorometer (excitation 380 nm, emission 450 nm).

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Boc-Val-Pro-Arg-AMC hydrochloride Related Antibodies
Molecular Weight

664.19

Formula

C31H46ClN7O7
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

CC(C(C(O1)=C2)=CC=C2NC([C@H](CCCNC(N)=N)NC([C@H](CCC3)N3C([C@H](C(C)C)NC(OC(C)(C)C)=O)=O)=O)=O)=CC1=O.Cl
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

H2O : 10 mg/mL (15.06 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.5056 mL 7.5280 mL 15.0559 mL
5 mM 0.3011 mL 1.5056 mL 3.0112 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 1.5056 mL 7.5280 mL 15.0559 mL 37.6398 mL
5 mM 0.3011 mL 1.5056 mL 3.0112 mL 7.5280 mL
10 mM 0.1506 mL 0.7528 mL 1.5056 mL 3.7640 mL
15 mM 0.1004 mL 0.5019 mL 1.0037 mL 2.5093 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Boc-Val-Pro-Arg-AMC hydrochloride Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Boc-Val-Pro-Arg-AMC70375-24-5Fluorescent DyeInhibitorinhibitorinhibit

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