2. Fluorescent Dye
  3. Fluorogenic Substrate
  4. Enzyme Labeling
  5. Protease Substrate

Protease Substrate

Protease Substrate (134):

Cat. No. Product Name CAS No. Purity Chemical Structure
  • HY-126839
    C12FDG 138777-25-0 99.84%
    C12FDG (5-Dodecanoylaminofluorescein di-β-D-Galactopyranoside) is a lipophilic green fluorescent substrate for β-galactosidase detection. C12-FDG is more sensitive than FDG (HY-101895) for beta-galactosidase activity determinations in animal cells.
    C12FDG
  • HY-15934
    X-GAL 7240-90-6 99.88%
    X-GAL (BCIG) is a widely used chromogenic β-galactosidase substrate. X-GAL is a colorless compound until cleaved by β-galactosidase, at which point X-GAL turns to an insoluble and detectable blue compound, making X-GAL particularly useful in techniques such as blue-white screening for cloning in bacteria. X-GAL can also be used for detection of β-galactosidase activity.
    X-GAL
  • HY-P1002
    Suc-Leu-Leu-Val-Tyr-AMC 94367-21-2 99.66%
    Suc-Leu-Leu-Val-Tyr-AMC is a fluorogenic substrate.
    Suc-Leu-Leu-Val-Tyr-AMC
  • HY-15902
    ABTS diammonium salt 30931-67-0 99.86%
    ABTS diammonium salt is a substrate for horseradish peroxidase (HRP) conjugate.
    ABTS diammonium salt
  • HY-W019823
    4-MUNANA 76204-02-9 98.08%
    4-MUNANA is a substrate of influenza virus neuraminidase (NA) with high selectivity and irreversible reaction. In the enzymatic reaction, 4-MUNANA is hydrolyzed by NA to generate fluorescent 4-methylumbelliferone (4-MU). By detecting the fluorescence intensity of 4-MU, quantitative analysis of NA activity can be achieved. 4-MUNANA can be used in influenza-related research, such as screening NA inhibitors, developing new anti-influenza drugs, and studying the infection mechanism of influenza viruses.
    4-MUNANA
  • HY-15925
    Nitro blue tetrazolium chloride 298-83-9 99.82%
    Nitro blue tetrazolium chloride (NBT) is a substrate for dehydrogenases; is used with the alkaline phosphatase substrate 5-Bromo-4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures.
    Nitro blue tetrazolium chloride
  • HY-D1027
    Dansylcadaverine 10121-91-2 98.69%
    Dansylcadaverine (Monodansyl cadaverine) is an autofluorescent compound used for the labeling of autophagic vacuoles. Dansylcadaverine, a high affinity substrate of transglutaminases, can block the receptor-mediated endocytosis of many ligands.
    Dansylcadaverine
  • HY-P0019A
    Z-Gly-Gly-Arg-AMC acetate 2070009-61-7 99.87%
    Z-Gly-Gly-Arg-AMC acetate is a thrombin-specific fluorogenic substrate for testing of thrombin generation in PRP and platelet-poor plasma (PPP).
    Z-Gly-Gly-Arg-AMC acetate
  • HY-P1003
    Ac-DEVD-AMC 169332-61-0 99.93%
    Ac-DEVD-AMC is a fluorescent substrate of caspase-3/caspase-7. When treating Ac-DEVD-AMC with cell lysate, Ac-DEVD-AMC releases amino-4-methylcoumarin (AMC) for fluorescence detection, with an excitation wavelength of 380 nm and an emission wavelength of 460 nm.
    Ac-DEVD-AMC
  • HY-101895
    Fluorescein di(β-D-galactopyranoside) 17817-20-8 99.81%
    Fluorescein di(β-D-galactopyranoside) is a fluorogenic substrate for β-galactosidase (λex=485 nm, λem=535 nm).
    Fluorescein di(β-D-galactopyranoside)
  • HY-D1670
    Z-Gly-Pro-AMC 68542-93-8 99.80%
    Z-Gly-Pro-AMC is a fluorogenic substrate. Z-Gly-Pro-AMC is hydrolyzed by prolyl endopeptidase to generate highly fluorescent 7-amido-4-methylcoumarin (HY-D0027). (λex=380 nm, λem=465 nm).
    Z-Gly-Pro-AMC
  • HY-137276
    5-Bromo-3-indolyl β-D-galactopyranoside 97753-82-7 99.73%
    5-Bromo-3-indolyl β-D-galactopyranoside (Bluo-Gal) is a chromogenic substrate for β-galactosidase. 5-Bromo-3-indolyl β-D-galactopyranoside is hydrolyzed by the enzyme to generate a 5-bromoindole intermediate, which is further oxidized to form an insoluble blue precipitate. 5-Bromo-3-indolyl β-D-galactopyranoside can specifically recognize bacterial β-galactosidases (such as the product of the Escherichia coli lacZ gene) and reacts at pH 7.4, making it suitable for light and electron microscopic observations. 5-Bromo-3-indolyl β-D-galactopyranoside can be used in histochemical detection of reporter gene expression in transgenic organisms, such as the localization analysis of β-galactosidase activity in mouse embryos or muscle tissues.
    5-Bromo-3-indolyl β-D-galactopyranoside
  • HY-137592
    Nicotinamide 1,N6-ethenoadenine dinucleotide 38806-38-1 ≥99.0%
    Nicotinamide 1,N6-ethenoadenine dinucleotide (ε-NAD) , a fluorescent analogue of NAD, is able to serve as a substrate for the bacterial toxincatalyzed G-ADP ribosylation of signal-transducing G-proteins. Nicotinamide 1,N6-ethenoadenine dinucleotide can be used as a fluorescent substrate for the studies of the ADP ribosylation reaction.
    Nicotinamide 1,N6-ethenoadenine dinucleotide
  • HY-P1005
    Ac-DEVD-AFC 201608-14-2 99.59%
    Ac-DEVD-AFC is a fluorogenic substrate (λex=400 nm, λem=530 nm).
    Ac-DEVD-AFC
  • HY-D0995
    7-Methyl-6-thioguanosine 55727-10-1 ≥99.0%
    7-Methyl-6-thioguanosine (MESG) is a chromophoric substrate which can be used for the quantitation of inorganic phosphate. 7-Methyl-6-thioguanosine is also used to determine the activity of purine nucleoside phosphorylase.
    7-Methyl-6-thioguanosine
  • HY-D0145
    7-Ethoxyresorufin 5725-91-7 99.61%
    7-Ethoxyresorufin (Resorufin ethyl ether) is a fluorometric substrate and competitive inhibitor of cytochrome P450, especially CYP1A1. 7-Ethoxyresorufin also inhibits NO synthase.
    7-Ethoxyresorufin
  • HY-W010991
    N-[3-(2-Furyl)acryloyl]-Phe-Gly-Gly 64967-39-1 99.59%
    N-[3-(2-Furyl)acryloyl]-Phe-Gly-Gly (FAPGG) is a specific substrate of angiotensin converting enzyme (ACE) with a Ki of 2.546×10-4 M. It is used as a chromogenic probe for quantitative detection of ACE activity. N-[3-(2-Furyl)acryloyl]-Phe-Gly-Gly can be hydrolyzed by ACE to generate N-[3-(2-furyl)acryloyl]-Phe (FAP) and Gly-Gly, and the ACE inhibitory effect is monitored by photometry. FAPGG competitively binds to the active center of ACE and is a key tool for screening ACE inhibitors such as Captopril (HY-B0368) and Dioscorin. Its reversible mechanism of action supports hypertension research and drug development targeting the renin-angiotensin system.
    N-[3-(2-Furyl)acryloyl]-Phe-Gly-Gly
  • HY-137845
    4-Methylumbelliferyl β-D-galactopyranoside 6160-78-7 99.06%
    4-Methylumbelliferyl β-D-galactopyranoside is a fluorescent substrate for β-galactosidase which, when cleaved, produces a water-soluble blue fluorescent coumarin fluorophore that can be detected using a fluoroenzymeter or fluorometer.
    4-Methylumbelliferyl β-D-galactopyranoside
  • HY-D0994
    4-Methylumbelliferyl phosphate 3368-04-5 99.63%
    4-Methylumbelliferyl phosphate (4-MUP), an anionic organophosphate, is a acid and alkaline phosphatase fluorogenic substrate. 4-Methylumbelliferyl phosphate is also a nerve agent simulant.
    4-Methylumbelliferyl phosphate
  • HY-137784
    Boc-Val-Pro-Arg-AMC hydrochloride 70375-24-5 99.43%
    Boc-Val-Pro-Arg-AMC hydrochloride is a sensitive fluorogenic substrate for measuring trypsin-like serine proteases activity.
    Boc-Val-Pro-Arg-AMC hydrochloride