Boc-Val-Pro-Arg-AMC 

Boc-Val-Pro-Arg-MCA is a sensitive fluorogenic substrate for measuring trypsin-like serine proteases activity^[1].

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Trypsin activity assay^[2]
1. Add diluted Trypsin enzyme (100 μL) to 800 μL of 50 mM Tris–HCl buffer (pH 8.0).
2. The reaction is immediately initiated by the addition of 100 μL of 50 μM substrate Boc-Val-Pro-Arg-MCA.
3. Incubate at 55 °C for 10 min.
4. Add 1.5 mL of the stopping agent (methyl alcohol:n-butyl alcohol:distilled water = 35:30:35, v/v/v) to stop the reaction.
5. Measure the fluorescence intensity by a spectrofluorometer (excitation 380 nm, emission 450 nm). Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

627.73

C₃₁H₄₅N₇O₇

65147-04-8

{Boc}-Val-Pro-Arg-{AMC}

{Boc}-VPR-{AMC}

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Liu JY, et al. Purification and characterization of a sarcoplasmic serine proteinase from threadfin bream Nemipterus virgatus muscle. Food Chem. 2019 Jun 30;284:198-204.  [Content Brief]

  [2]. Sriket C, et al. Low molecular weight trypsin from hepatopancreas of freshwater prawn (Macrobrachium rosenbergii): Characteristics and biochemical properties. Food Chemistry, 2012, 134(1): 351-358.