2. Fluorescent Dye
  3. Fluorogenic Substrate

Fluorogenic Substrate

 

Fluorogenic Substrate (183):

Cat. No. Product Name CAS No. Purity Chemical Structure
  • HY-12591B
    D-Luciferin potassium 115144-35-9 99.97%
    D-luciferin is the natural substrate of the enzyme luciferase (Luc) that catalyzes the production of the typical yellowgreen light of fireflies. The 560 nm chemiluminescence from this reaction peaks within seconds, with light output that is proportional to luciferase concentration when the substrate luciferin is present in excess. The luciferase (luc) gene is a popular reporter gene for research and agent screening. Chemiluminescent techniques are virtually background-free, making the luc reporter gene ideal for detecting low-level gene expression. As little as 0.02 pg of luciferase can be reliably measured in a standard scintillation counter. In addition to its role as a reporter of gene expression, luciferase is commonly used in an extremely sensitive assay for ATP. We offer the firefly luciferase (HY-P1004), luciferin free acid (HY-12591A), as well as its water-soluble sodium salts (HY-12591) and potassium salts (HY-12591B) .
    D-Luciferin potassium
  • HY-12591A
    D-Luciferin 2591-17-5 99.87%
    D-luciferin is the natural substrate of the enzyme luciferase (Luc) that catalyzes the production of the typical yellowgreen light of fireflies. The 560 nm chemiluminescence from this reaction peaks within seconds, with light output that is proportional to luciferase concentration when the substrate luciferin is present in excess. The luciferase (luc) gene is a popular reporter gene for research and agent screening. Chemiluminescent techniques are virtually background-free, making the luc reporter gene ideal for detecting low-level gene expression. As little as 0.02 pg of luciferase can be reliably measured in a standard scintillation counter. In addition to its role as a reporter of gene expression, luciferase is commonly used in an extremely sensitive assay for ATP. We of er the firefly luciferase (HY-P1004), luciferin free acid (HY-12591A), as well as its water-soluble sodium salts (HY-12591) and potassium salts (HY-12591B) .
    D-Luciferin
  • HY-12591
    D-Luciferin sodium 103404-75-7 99.92%
    D-luciferin is the natural substrate of the enzyme luciferase (Luc) that catalyzes the production of the typical yellowgreen light of fireflies. The 560 nm chemiluminescence from this reaction peaks within seconds, with light output that is proportional to luciferase concentration when the substrate luciferin is present in excess. The luciferase (luc) gene is a popular reporter gene for research and agent screening. Chemiluminescent techniques are virtually background-free, making the luc reporter gene ideal for detecting low-level gene expression. As little as 0.02 pg of luciferase can be reliably measured in a standard scintillation counter. In addition to its role as a reporter of gene expression, luciferase is commonly used in an extremely sensitive assay for ATP. We of er the firefly luciferase (HY-P1004), luciferin free acid (HY-12591A), as well as its water-soluble sodium salts (HY-12591) and potassium salts (HY-12591B) .
    D-Luciferin sodium
  • HY-126839
    C12FDG 138777-25-0 99.84%
    C12FDG (5-Dodecanoylaminofluorescein di-β-D-Galactopyranoside) is a lipophilic green fluorescent substrate for β-galactosidase detection. C12-FDG is more sensitive than FDG (HY-101895) for beta-galactosidase activity determinations in animal cells.
    C12FDG
  • HY-18743
    Coelenterazine 55779-48-1 98.65%
    Coelenterazine is a luminescent enzyme substrate for apoaequorin and Renilla luciferase. Renilla luciferase and substrate coelenterazine has been used as the bioluminescence donor in bioluminescence resonance energy transfer (BRET) to detect protein-protein interactions. Coelenterazine is a superoxide anion-sensitive chemiluminescent probe and it can also be used in chemiluminescent detection of peroxynitrite.
    Coelenterazine
  • HY-32264
    X-Neu5Ac sodium 160369-85-7 99.98%
    X-Neu5Ac (sodium) is a substrate for chromogenic assay of neuraminidase activity in bacterial expression systems; with a Km of 0.89 mM for neuraminidase.
    X-Neu5Ac sodium
  • HY-W011422
    2,6-Dibromo-4-(chloroimino)cyclohexa-2,5-dienone 537-45-1
    2,6-Dibromo-4-(chloroimino)cyclohexa-2,5-dienone (NSC 528) is a colorimetric dye used for the detection of phenolic compounds. 2,6-Dibromo-4-(chloroimino)cyclohexa-2,5-dienone reacts with phenolic compounds at a pH of about 9.4 to produce an indigo dye. 2,6-Dibromo-4-(chloroimino)cyclohexa-2,5-dienone has been used as a Gibbs reagent for the colorimetric detection of phenolic compounds. 2,6-Dibromo-4-(chloroimino)cyclohexa-2,5-dienone has also been used in a colorimetric reaction for the quantitative colorimetric detection of aflatoxins, turning the colorless aflatoxins green with an absorption band at 673 nm.
    2,6-Dibromo-4-(chloroimino)cyclohexa-2,5-dienone
  • HY-100045
    p-Nitrophenyl phosphorylcholine 21064-69-7
    p-Nitrophenyl phosphorylcholine (4-Nitrophenylphosphorylcholine) is a chromogenic substrate that is used to measure phospholipase C (PLC) activity. Hydrolysis of p-nitrophenylphosphorylcholine by PLC results in the liberation of p-nitrophenol, which can be measured at 405 nm at pH 7.2-7.5.
    p-Nitrophenyl phosphorylcholine
  • HY-111382
    Diphenylterazine 344940-63-2 98.93%
    Diphenylterazine (DTZ) is a bioluminescence agent. Diphenylterazine alone yielded very little background, leading to excellent signal-to-background ratios.
    Diphenylterazine
  • HY-15934
    X-GAL 7240-90-6 99.88%
    X-GAL (BCIG) is a widely used chromogenic β-galactosidase substrate. X-GAL is a colorless compound until cleaved by β-galactosidase, at which point X-GAL turns to an insoluble and detectable blue compound, making X-GAL particularly useful in techniques such as blue-white screening for cloning in bacteria. X-GAL can also be used for detection of β-galactosidase activity.
    X-GAL
  • HY-P1002
    Suc-Leu-Leu-Val-Tyr-AMC 94367-21-2 99.57%
    Suc-Leu-Leu-Val-Tyr-AMC is a fluorogenic substrate.
    Suc-Leu-Leu-Val-Tyr-AMC
  • HY-15902
    ABTS diammonium salt 30931-67-0 99.86%
    ABTS diammonium salt is a substrate for horseradish peroxidase (HRP) conjugate.
    ABTS diammonium salt
  • HY-15925
    Nitro blue tetrazolium chloride 298-83-9 99.82%
    Nitro blue tetrazolium chloride (NBT) is a substrate for dehydrogenases; is used with the alkaline phosphatase substrate 5-Bromo-4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures.
    Nitro blue tetrazolium chloride
  • HY-D1027
    Dansylcadaverine 10121-91-2 98.62%
    Dansylcadaverine (Monodansyl cadaverine) is an autofluorescent compound used for the labeling of autophagic vacuoles. Dansylcadaverine, a high affinity substrate of transglutaminases, can block the receptor-mediated endocytosis of many ligands.
    Dansylcadaverine
  • HY-111653
    CycLuc1 1247879-16-8 99.72%
    CycLuc1 is a blood-brain barrier permeable luciferase substrate that displays near-infrared (NIR) emission with a peak luminescence wavelength of 599 nm. CycLuc1 can be used for in vivo bioluminescence imaging.
    CycLuc1
  • HY-W019823
    4-MUNANA 76204-02-9 98.08%
    4-MUNANA is a fluorescent substrate used for neuraminidase activity assay.
    4-MUNANA
  • HY-N2306
    Aclacinomycin A 57576-44-0 99.30%
    Aclacinomycin A (Aclarubicin) is an orally active and potent anthracycline antitumor antibiotic. Aclacinomycin A is an inhibitor of topoisomerase I and II. Aclacinomycin A inhibits synthesis of nucleic acid, especially RNA. Aclacinomycin A might inhibit the 26S protease complex as well as the ubiquitin-ATP-dependent proteolysis.
    Aclacinomycin A
  • HY-P0019A
    Z-Gly-Gly-Arg-AMC acetate 2070009-61-7 99.87%
    Z-Gly-Gly-Arg-AMC acetate is a thrombin-specific fluorogenic substrate for testing of thrombin generation in PRP and platelet-poor plasma (PPP).
    Z-Gly-Gly-Arg-AMC acetate
  • HY-P1003
    Ac-DEVD-AMC 169332-61-0 99.93%
    Ac-DEVD-AMC is a fluorescent substrate of caspase-3/caspase-7. When treating Ac-DEVD-AMC with cell lysate, Ac-DEVD-AMC releases amino-4-methylcoumarin (AMC) for fluorescence detection, with an excitation wavelength of 380 nm and an emission wavelength of 460 nm.
    Ac-DEVD-AMC
  • HY-101895
    Fluorescein di(β-D-galactopyranoside) 17817-20-8 99.81%
    Fluorescein di(β-D-galactopyranoside) is a fluorogenic substrate for β-galactosidase (λex=485 nm, λem=535 nm).
    Fluorescein di(β-D-galactopyranoside)