1. Recombinant Proteins
  2. Enzymes & Regulators
  3. Isomerases (EC 5)
  4. DPEase Protein, Ruminiclostridium cellulolyticum (FLAG, His)

DPEase Protein, Ruminiclostridium cellulolyticum (FLAG, His)

Cat. No.: HY-P702112
Handling Instructions

DPEase Protein orchestrates D-fructose epimerization at the C3 position, crucial in D-psicose biosynthesis. Its remarkable specificity ensures precise sugar formation, particularly with D-psicose. Notably, minimal activity with D-tagatose underscores the enzyme's discerning nature and limited reactivity to this substrate. DPEase Protein, Ruminiclostridium cellulolyticum (FLAG, His) is the recombinant DPEase protein, expressed by E. coli , with N-6*His, N-Flag labeled tag. The total length of DPEase Protein, Ruminiclostridium cellulolyticum (FLAG, His) is 293 a.a., .

For research use only. We do not sell to patients.

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  • Biological Activity

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Description

DPEase Protein orchestrates D-fructose epimerization at the C3 position, crucial in D-psicose biosynthesis. Its remarkable specificity ensures precise sugar formation, particularly with D-psicose. Notably, minimal activity with D-tagatose underscores the enzyme's discerning nature and limited reactivity to this substrate. DPEase Protein, Ruminiclostridium cellulolyticum (FLAG, His) is the recombinant DPEase protein, expressed by E. coli , with N-6*His, N-Flag labeled tag. The total length of DPEase Protein, Ruminiclostridium cellulolyticum (FLAG, His) is 293 a.a., .

Background

The DPEase Protein plays a pivotal role in the biosynthesis of D-psicose, orchestrating the reversible epimerization of D-fructose at the C3 position to yield D-psicose. This enzyme demonstrates a remarkable specificity for D-psicose, highlighting its essential contribution to the precise formation of this sugar. Notably, the enzyme exhibits minimal activity with D-tagatose, underscoring its discerning nature and limited reactivity towards this particular substrate.

Species

Others

Source

E. coli

Tag

N-6*His;N-Flag

Accession

B8I944 (M1-S293)

Gene ID

/

Molecular Construction
N-term
Flag-6*His
DPEase (M1-S293)
Accession # B8I944
C-term
Synonyms
D-psicose 3-epimerase; DPEase
Purity

Greater than 90% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

DPEase Protein, Ruminiclostridium cellulolyticum (FLAG, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
DPEase Protein, Ruminiclostridium cellulolyticum (FLAG, His)
Cat. No.:
HY-P702112
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