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  2. Recombinant Antibody Expression Service

Recombinant Antibody Expression Service

Recombinant antibodies are genetically engineered to generate specific antibodies within host cells. They are commonly utilized in medical research, diagnostics, and therapeutics. In comparison with traditional hybridoma antibodies, recombinant antibodies can be produced rapidly and in large quantities, featuring higher specificity and consistency. This is achieved by directly employing antibody gene sequences without the need for immunization and screening.

Utilizing a mammalian cell recombinant expression platform, MCE has devised practical methods and tools for expressing recombinant monoclonal antibodies targeting antibody sequences, aiming to achieve high yields, high purity, and low endotoxin levels. In addition to expressing full-length antibodies, MCE can also produce various types of recombinant antibodies, such as Fab, scFv, VHH, fusion proteins, chimeric antibodies, and bispecific antibodies. MCE's recombinant antibody expression services encompass codon optimization, vector construction, expression, purification, and QC analysis. All you need to do is provide the sequence information of the antibody (or VH/VL) to MCE. We are dedicated to providing high-quality recombinant antibodies to assist your research endeavors.

General Workflow of Antibody Expression Services
Starting with Sequence information
Gene synthesis with Codon, optimization, vector construction
Expression in Mammalian Cell

(CHO, HEK293, FUT8-KO CHO)

Purification

(Protein A, Protein G, Protein L, IEX, SEC, HIC)

QC Testing: A280, Purity >95%

(SDS-PAGE and SEC-HPLC), Endotoxin <1 EU/mg

Delivery: Recombinant Antibodies
Your Trusted Partner for Antibody Services
High reproducibility
Defined by amino acid sequences to ensure reproducibility
High speed(As fast as 2 weeks)
Fast delivery from genes to antibodies
Flexible production scales
mg to g level
Multiple antibody forms available
IgG, scFv, Fab, BiTE, VHH, etc.

Detailed QC Data Available

ELISA (EC50), LC-MS, FACS, SPR, BLI, Fc - effector function assays,etc.

Featured Case Studies

Case1. IgG1

Reozalimab expressed in CHO cells

Purity>95% as analyzed by SDS-PAGE under reducing (R) and
non-reducing (N) condition
Reozalimab is free of aggregation and has a purity of up to 100.00%, determined by SEC-HPLC.

Case2 .Bispecific Antibody

Amivantamab expressed in CHO cells

Purity >95% as analyzed by SDS-PAGE under reducing (R) and non-reducing (N) condition.
Amivantamab is free of aggregation and has a purity of up to 100.00%, determined by SEC-HPLC.
The Deglycosylated masses are detected by LC-MS. The measured masses are consistent with theoretical masses.
Immobilized Human c-MET/HGFR Protein (ECD, His Tag) can bind Amivantamab. The EC50 for this effect is 1.20 ng/mL.

Case3. Fusion Protein

Retlirafusp alfa expressed in CHO cells

Purity>95% as analyzed by SDS-PAGE under reducing (R) and non-reducing (N) condition.
Retlirafusp alfa is free of aggregation and has a purity of up to 100.00%, determined by SEC-HPLC.
Free Quote for Custom Recombinant Antibody Expression Services
For Further information on service options and corresponding prices, please enter the required information below or contact us directly at 609-228-6898
or E-mail at sales@MedChemExpress.com.
* Antibody Name: * Sequence:
Subtype selection for antibody backbone (optional): * Amount:
* Expression System: * Purity:
* Application:
* Organization Name: * Email Address:
* Phone Number: * Research Field:
* Country or Region:    
Remarks: