Fusion glycoprotein F0 Protein, Measles virus (Cell-Free, His, Avi)

Fusion glycoprotein F0 directs the fusion of viral and plasma cell membranes, transitioning between prefusion, prehairpin, and postfusion states. Fusion glycoprotein F0 Protein, Measles virus (Cell-Free, His, Avi) is the recombinant Virus-derived Fusion glycoprotein F0 protein, expressed by E. coli Cell-free , with N-10*His, C-Avi labeled tag. The total length of Fusion glycoprotein F0 Protein, Measles virus (Cell-Free, His, Avi) is 527 a.a., with molecular weight of 61.8 kDa.

The Fusion glycoprotein F0 protein, classified as a Class I viral fusion protein, exhibits a dynamic conformational repertoire encompassing at least three states: the pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During the fusion events involving viral and plasma cell membranes, the heptad repeat (HR) regions adopt a trimer-of-hairpins structure, placing the fusion peptide in close proximity to the C-terminal region of the ectodomain. This configuration appears pivotal in driving the apposition and subsequent fusion of the viral and plasma cell membranes. The Fusion glycoprotein F0 protein plays a central role in directing the fusion of viral and cellular membranes, facilitating the delivery of the nucleocapsid into the cytoplasm. Notably, this fusion process is pH independent and occurs directly at the outer cell membrane. The trimeric assembly of F1-F2 (F protein) likely interacts with H at the virion surface. Upon HN binding to its cellular receptor, the hydrophobic fusion peptide is exposed, initiating interaction with the cellular membrane and inducing fusion between the cell and virion membranes. In later stages of infection, F proteins expressed at the plasma membrane of infected cells may mediate fusion with adjacent cells, leading to syncytia formation, a cytopathic effect that could contribute to tissue necrosis. The Fusion glycoprotein F0 protein exists as a homotrimer of disulfide-linked F1-F2 units.

Virus

E. coli Cell-free

N-10*His;C-Avi

P69356 (Q24-L550)

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QIHWGNLSKIGVVGIGSASYKVMTRSSHQSLVIKLMPNITLLNNCTRVEIAEYRRLLRTVLEPIRDALNAMTQNIRPVQSVASSRRHKRFAGVVLAGAALGVATAAQITAGIALHQSMLNSQAIDNLRASLETTNQAIEAIRQAGQEMILAVQGVQDYINNELIPSMNQLSCDLIGQKLGLKLLRYYTEILSLFGPSLRDPISAEISIQALSYALGGDINKVLEKLGYSGGDLLGILESRGIKARITHVDTESYFIVLSIAYPTLSEIKGVIVHRLEGVSYNIGSQEWYTTVPKYVATQGYLISNFDESSCTFMPEGTVCSQNALYPMSPLLQECLRGSTKSCARTLVSGSFGNRFILSQGNLIANCASILCKCYTTGTIINQDPDKILTYIAADHCPVVEVNGVTIQVGSRRYPDAVYLHRIDLGPPISLERLDVGTNLGNAIAKLEDAKELLESSDQILRSMKGLSSTSIVYILIAVCLGGLIGIPALICCCRGRCNKKGEQVGMSRPGLKPDLTGTSKSYVRSL

61.8 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.