2. Recombinant Proteins
  3. Others
  4. NLRP5 Protein, Human (Sf9, His, Strep, MBP)

NLRP5 Protein, Human (Sf9, His, Strep, MBP)

Cat. No.: HY-P702004
Handling Instructions

NLRP5 is a key SCMC member that coordinates zygote progression by regulating the formation of F-actin CPL, ensuring symmetric division and spindle positioning. It directs cortical granule positioning, connects to the actin scaffold for granule localization, and contributes to actin clearance prior to oocyte exocytosis. NLRP5 Protein, Human (Sf9, His, Strep, MBP) is the recombinant human-derived NLRP5 protein, expressed by Sf9 insect cells , with N-Strep, N-MBP, N-8*His labeled tag. The total length of NLRP5 Protein, Human (Sf9, His, Strep, MBP) is 974 a.a., .

For research use only. We do not sell to patients.

Surface Plasmon Resonance (SPR) Assay Service   Protein Expression Service

Size Stock
50 μg   Get quote  
100 μg   Get quote  

* Please select Quantity before adding items.

Top Publications Citing Use of Products

  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

NLRP5 is a key SCMC member that coordinates zygote progression by regulating the formation of F-actin CPL, ensuring symmetric division and spindle positioning. It directs cortical granule positioning, connects to the actin scaffold for granule localization, and contributes to actin clearance prior to oocyte exocytosis. NLRP5 Protein, Human (Sf9, His, Strep, MBP) is the recombinant human-derived NLRP5 protein, expressed by Sf9 insect cells , with N-Strep, N-MBP, N-8*His labeled tag. The total length of NLRP5 Protein, Human (Sf9, His, Strep, MBP) is 974 a.a., .
Background

NLRP5, a vital component of the subcortical maternal complex (SCMC), plays a crucial role in facilitating the progression of zygotes beyond the initial embryonic cell divisions by regulating actin dynamics. Its involvement in the formation of F-actin cytoplasmic lattices (CPL) in oocytes is essential for ensuring the symmetric division of zygotes through the regulation of mitotic spindle formation and positioning. Additionally, NLRP5 is required for the localization of cortical granules to the oocyte cortex, achieved through its association with the cortical actin scaffold. Furthermore, it contributes to cortical actin clearance preceding oocyte exocytosis. NLRP5 is implicated in the regulation of post-fertilization Ca(2+) release and endoplasmic reticulum (ER) storage by influencing ER localization. Its potential involvement in the localization of mitochondria in oocytes and early embryos, independent of CPL formation, adds another layer to its multifaceted functions. Within the SCMC, NLRP5 interacts with other components such as OOEP, KHDC3, and TLE6, and may facilitate the translocation of these components between nuclear and cytoplasmic compartments. Additionally, NLRP5 interacts with PRKCE and TUBB3, highlighting its diverse molecular associations.
Species

Human
Source

Sf9 insect cells
Tag

N-Strep;N-MBP;N-8*His
Accession

P59047 (G227-N1200)
Gene ID

126206
Molecular Construction
N-term
8*His-Strep-MBP
NLRP5 (G227-N1200)
Accession # P59047
C-term
Synonyms
NLRP5; NACHT; LRR and PYD domains-containing protein 5; Mater protein homolog; Maternal Antigen that Embryos Require
Purity

Greater than 90% as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

NLRP5 Protein, Human (Sf9, His, Strep, MBP) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Salutation

Applicant Name *

 

Email Address *

Phone Number *

 

Organization Name *

Department *

 

Requested quantity *

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
NLRP5 Protein, Human (Sf9, His, Strep, MBP)
Cat. No.:
HY-P702004
Quantity:
MCE Japan Authorized Agent:

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Country or Region *

Email Address *

     

Phone Number *

 

Organization Name *

Remarks

SAFETY DATA SHEET (SDS)

English - EN (0 KB) Français - FR (0 KB) Deutsch - DE (0 KB) Norwegian - NO (0 KB) Español - ES (0 KB) Swedish - SV (0 KB)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.