CYP3A4 Protein, Human (His)

The CYP3A4 protein, encoded by the CYP3A4 gene, is a key enzyme in drug metabolism and the synthesis of cholesterol, steroids, and lipids. It localizes to the endoplasmic reticulum and is induced by glucocorticoids and certain drugs. CYP3A4 Protein, Human (His) is the recombinant human-derived CYP3A4 protein, expressed by E. coli , with N-His labeled tag. The total length of CYP3A4 Protein, Human (His) is 502 a.a., with molecular weight of ~59.2 kDa.

The CYP3A4 gene encodes a member of the cytochrome P450 superfamily of enzymes, which are monooxygenases playing a crucial role in drug metabolism and the synthesis of cholesterol, steroids, and lipids. This enzyme localizes to the endoplasmic reticulum, and its expression is induced by glucocorticoids and certain pharmacological agents. CYP3A4 is a key player in the metabolism of about half of the drugs in current use, encompassing substances like acetaminophen, codeine, cyclosporin A, diazepam, erythromycin, and chloroquine. Additionally, the enzyme participates in the metabolism of steroids and carcinogens. The gene is situated in a cluster of cytochrome P450 genes on chromosome 7q21.1. Formerly, another CYP3A gene, CYP3A3, was believed to exist, but it is now considered to be a transcript variant of CYP3A4. Multiple alternatively spliced transcript variants have been identified, encoding different isoforms of the enzyme. With biased expression observed in the liver (RPKM 476.5), small intestine (RPKM 282.8), and one other tissue, CYP3A4 is a pivotal factor in drug metabolism and overall xenobiotic processing.

Human

E. coli

N-His

NP_059488.2 (A2-A503)

1576  [NCBI]

Approximately 59.2 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.