AF488 azide 

AF488 azide is a fluorescent dye that can be used to determine the reactivity of immobilized DIBO groups. It contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups. AF488 exhibits the λ_em and λ_ex wavelength of 520 nm and 470 nm, respectively^[1][2].

692.76

C₂₄H₁₈K₂N₆O₁₀S₂

Solid

Brown to red

O=C(C1=C(C2=C(C=CC(N)=C3S(=O)(O[K])=O)C3=[O+]C4=C2C=CC(N)=C4S(=O)([O-])=O)C=CC(C(NCCCN=[N+]=[N-])=O)=C1)O[K]

Room temperature in continental US; may vary elsewhere.

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

• [1]. Ruff SM, et al. clickECM: Development of a cell-derived extracellular matrix with azide functionalities. Acta Biomater. 2017 Apr 1;52:159-170.  [Content Brief]

  [2]. Chakraborty S, Hu S Y, Wu S H, et al. The interaction affinity between vascular cell adhesion molecule-1 (VCAM-1) and very late antigen-4 (VLA-4) analyzed by quantitative FRET[J]. PLoS One, 2015, 10(3): e0121399.