2. Academic Validation
  3. Induction and mechanism of HeLa cell apoptosis by 9-oxo‑10, 11-dehydroageraphorone from Eupatorium adenophorum

Induction and mechanism of HeLa cell apoptosis by 9-oxo‑10, 11-dehydroageraphorone from Eupatorium adenophorum

  • Oncol Rep. 2015 Apr;33(4):1823-7. doi: 10.3892/or.2015.3778.
Fei Liao 1,  Yanchun Hu 1,  Lei Wu 1,  Hui Tan 1,  Biao Luo 1,  Yajun He 1,  Yan Qiao 1,  Quan Mo 1,  Ya Wang 1,  Zhicai Zuo 1,  Junliang Deng 1,  Yahui Wei 2
Affiliations

Affiliations

  • 1 Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Sichuan, Wenjiang 611130, P.R. China.
  • 2 Key Laboratory of Resource Biology and Biotechnology in Western China, School of Life Science, Northwest University, Xi'an 710069, P.R. China.
PMID: 25647450 DOI: 10.3892/or.2015.3778
Abstract

9-Oxo-10, 11-dehydroageraphorone (euptox A), a cadenine sesquiterpene, is the main toxin from Eupatorium adenophorum. The aim of the present study was to examine the induction and mechanism of HeLa cell Apoptosis by euptox A. The apoptosis‑inducing effect of the euptox A on HeLa cells was examined by MTT assay. The underlying mechanism was analyzed by flow cytometry and quantitative PCR. Flow cytometry results suggested that euptox A effectively inhibited the proliferation of HeLa cells, arrested the cell cycle transition from S to G2/M phase, did not continue to complete the cell cycle activity (mainly from 4 times and mitosis), and induced cell proliferation. The RT-qPCR detection results showed that euptox A induced Apoptosis by improving the gene expression level of apoptotic proteases such as Caspase-10 in HeLa cells. Its mechanism of action was associated with the upregulation of apoptotic gene expression and arresting of the cell cycle.

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