2. Academic Validation
  3. Phosphatase-mimicking Zr@PDA nanozyme with excellent dispersion stability for the detection of fructose 1,6-diphosphate

Phosphatase-mimicking Zr@PDA nanozyme with excellent dispersion stability for the detection of fructose 1,6-diphosphate

  • Talanta. 2024 Nov 1:279:126657. doi: 10.1016/j.talanta.2024.126657.
Yusha Huang 1,  Chengli Xie 1,  Longcheng Gong 1,  Min Wang 2,  Lianzhe Hu 3,  Zhining Xia 1
Affiliations

Affiliations

  • 1 Chongqing Key Laboratory of Natural Product Synthesis and Drug Research, Innovative Drug Research Center, School of Pharmaceutical Sciences, Chongqing University, Chongqing, 401331, China.
  • 2 Chongqing Key Laboratory of Natural Product Synthesis and Drug Research, Innovative Drug Research Center, School of Pharmaceutical Sciences, Chongqing University, Chongqing, 401331, China. Electronic address: wang_min@cqu.edu.cn.
  • 3 Chongqing Key Laboratory of Green Synthesis and Applications, College of Chemistry, Chongqing Normal University, Chongqing, 401331, China. Electronic address: lianzhehu@cqnu.edu.cn.
PMID: 39111218 DOI: 10.1016/j.talanta.2024.126657
Abstract

Zr4+-doped polydopamine (Zr@PDA) nanozyme with phosphatase-like activity was synthesized by a one-pot hydrothermal method for the first time. Compared with previous representative phosphatase-mimicking nanozymes (i.e., CeO2 NPs, ZrO2 NPs and UiO-66), Zr@PDA not only exhibited higher dispersion stability in water, but also higher catalytical efficiency. Kcat/Km of Zr@PDA is 35 and 12 times that of UiO-66 and ZrO2 NPs, respectively, which would endow the Zr@PDA-based analytical methods with high sensitivity. As a demonstration, a novel colorimetric method based on Zr@PDA nanozyme was developed for sensitive detection of the drug fructose 1,6-diphosphate. The linear range is 1-15 μM with a detection limit as low as 0.38 μM.

Keywords

Colorimetry; Dispersion stability; Fructose 1,6-diphosphate; Hydrolysis; Nanozyme.

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