2. Recombinant Proteins
  3. Cytokines and Growth Factors
  4. VEGF & VEGFR
  5. VEGF
  6. VEGF-A
  7. VEGF165 Protein, zebrafish (sf9)

VEGF165 Protein, zebrafish (sf9)

Cat. No.: HY-P74476
Handling Instructions

The VEGF165 protein is an important growth factor that actively drives vasculogenesis, vasculogenesis, and endothelial cell growth. Its multiple effects include inducing endothelial cell proliferation, promoting migration, inhibiting apoptosis, and increasing vascular permeability. VEGF165 Protein, zebrafish (sf9) is the recombinant VEGF165 protein, expressed by Sf9 insect cells , with tag free.

For research use only. We do not sell to patients.

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Description

The VEGF165 protein is an important growth factor that actively drives vasculogenesis, vasculogenesis, and endothelial cell growth. Its multiple effects include inducing endothelial cell proliferation, promoting migration, inhibiting apoptosis, and increasing vascular permeability. VEGF165 Protein, zebrafish (sf9) is the recombinant VEGF165 protein, expressed by Sf9 insect cells , with tag free.
Background

The VEGF165 Protein, a crucial growth factor, actively participates in angiogenesis, vasculogenesis, and endothelial cell growth. Its diverse effects include inducing endothelial cell proliferation, promoting cell migration, inhibiting apoptosis, and inducing the permeabilization of blood vessels. VEGF165 acts both upstream of kdr and tie1 to stimulate endothelial cell differentiation and upstream of gata1 to stimulate hematopoietic cell differentiation. Structurally, it exists as a homodimer linked by disulfide bonds. The isoform VEGF165 specifically binds to kdr and kdrl, highlighting its central role in orchestrating multiple cellular processes critical for vascular development and maintenance.
Species

Others
Source

Sf9 insect cells
Tag

Tag Free
Accession

O73682-1 (A24-R188)
Gene ID

30682  [NCBI]
Molecular Construction
N-term
VEGF165 (A24-R188)
Accession # O73682-1
C-term
Synonyms
VEGF-AA; Vascular endothelial growth factor A-A; vegf; vegfa
Molecular Weight

Approximately 22 kDa
Purity

Greater than 90% as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

VEGF165 Protein, zebrafish (sf9) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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