LPd peroxida probe 

LPd peroxida probe, a marker of ferroptosis, is a useful fluorescent probe for investigating the roles of lipid peroxidation in a variety of cell pathophysiologies. LPd peroxida probe reduces lipid hydroperoxides to lipid alcohols and is used for imaging lipid hydroperoxides in living cells^[1][2][3].

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. Cells in the glass bottom dish are treated with 50 µM of H₂O₂ in RPMI 1640 for 2 h at 37 °C. After removing H₂O₂, cells are washed with Hank’s balanced salt solution (HBSS) three times.
2. Cells are then treated with 10 µM of LPd peroxida probe in HBSS for 30 min at 37 °C. After removing LPd peroxida probe, cells are washed with HBSS three times.
3. Fluorescence images are obtained using a BZ-8000 fluorescence microscope from 3 separate dishes for each treatment^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

840.85

C₅₁H₄₁N₂O₈P

1448846-35-2

Solid

Pink to red

535

524

O=C1C2=CC=C3C4=CC=C5C(N(CCOCCOCCOCCOC)C(C6=CC=C(C4=C56)C7=C3C2=C(C(N1C8=CC=C(P(C9=CC=CC=C9)C%10=CC=CC=C%10)C=C8)=O)C=C7)=O)=O

Room temperature in continental US; may vary elsewhere.

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

• [1]. Kazuo Tomita, et al. MiR-7-5p Is Involved in Ferroptosis Signaling and Radioresistance Thru the Generation of ROS in Radioresistant HeLa and SAS Cell Lines. Int J Mol Sci. 2021 Aug 2;22(15):8300.  [Content Brief]

  [2]. Elizabeth M Kenny, et al. Ferroptosis Contributes to Neuronal Death and Functional Outcome After Traumatic Brain Injury. Crit Care Med. 2019 Mar;47(3):410-418.  [Content Brief]

  [3]. Kazunori Yamanaka, et al. A novel fluorescent probe with high sensitivity and selective detection of lipid hydroperoxides in cells. RSC Advances, 2012, 2, 7894–7900.