Granzyme B/GZMB Protein, Human (HEK293, His)

Granzyme B (GZMB) is a cytosolic protease enriched in cytotoxic T cells and natural killer cells and is critical for immune defense. Upon delivery to target cells, GZMB activates caspase-independent pyroptosis by cleaving gasdermin-E (GSDME). Granzyme B/GZMB Protein, Human (HEK293, His) is the recombinant human-derived Granzyme B/GZMB protein, expressed by HEK293 , with C-His labeled tag.

Granzyme B (GZMB) is a highly abundant protease residing in the cytosolic granules of cytotoxic T-cells and natural killer cells, playing a pivotal role in immune defense mechanisms. Upon delivery into the target cell through the immunological synapse, GZMB activates caspase-independent pyroptosis by catalyzing the cleavage of gasdermin-E (GSDME). This results in the release of the pore-forming moiety of GSDME, triggering pyroptosis and ultimately leading to target cell death. GZMB exhibits a substrate specificity for cleavage after aspartate residues. Additionally, it is implicated in an activation cascade of caspases, including caspase-3, -9, and -10, thereby contributing to apoptosis execution. Moreover, GZMB is involved in the response to bacterial infection, where it cleaves and activates caspase-7, promoting plasma membrane repair. The multifaceted activities of GZMB highlight its crucial role in orchestrating immune responses and cellular defense mechanisms.

Measured by its ability to cleave a peptide substrate, t-Butyloxycaronyl-Ala-Ala-Asp-ThioBenzylester (Boc-AAD-SBzl), in the presence of 5,5’-Dithio-bis (2-nitrobenzoic acid) (DTNB) and the specific activity is ≥1500 pmol/min/μg. Note: The enzyme needs to be activated by Active Cathepsin C.

Human

HEK293

C-His

NP_004122.1 (G19-Y247)

3002  [NCBI]

35-40 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.