2. Recombinant Proteins
  3. Enzymes & Regulators
  4. Hydrolases (EC 3)
  5. nitA Protein, Rhodococcus rhodochrous

nitA Protein, Rhodococcus rhodochrous

Cat. No.: HY-P702183
Handling Instructions

nitA protein enzymatically acts on aliphatic nitriles, specifically acrylonitrile, crotononitrile, and glutaronitrile substrates. nitA Protein, Rhodococcus rhodochrous is the recombinant nitA protein, expressed by E. coli , with tag free. The total length of nitA Protein, Rhodococcus rhodochrous is 369 a.a., .

For research use only. We do not sell to patients.

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Description

nitA protein enzymatically acts on aliphatic nitriles, specifically acrylonitrile, crotononitrile, and glutaronitrile substrates. nitA Protein, Rhodococcus rhodochrous is the recombinant nitA protein, expressed by E. coli , with tag free. The total length of nitA Protein, Rhodococcus rhodochrous is 369 a.a., .
Background

nitA protein demonstrates its enzymatic activity on aliphatic nitriles, specifically targeting substrates like acrylonitrile, crotononitrile, and glutaronitrile.
Species

Others
Source

E. coli
Tag

Tag Free
Accession

Q02068 (M1-K369)
Gene ID

/
Molecular Construction
N-term
nitA (M1-K369)
Accession # Q02068
C-term
Synonyms
Aliphatic nitrilase
Purity

Greater than 90% as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

nitA Protein, Rhodococcus rhodochrous Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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