2. Membrane Transporter/Ion Channel Neuronal Signaling GPCR/G Protein
  3. Potassium Channel 5-HT Receptor
  4. Spadin

Spadin, a natural peptide derived from a propeptide released in blood, is a potent TREK-1 channel blocker with IC50 value of 10 nM. Spadin enhances dorsal raphe nucleus 5-HT neurotransmission in mice and induces hippocampal CREB activation and neurogenesis. Spadin can be used for antidepressant research.

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Custom Peptide Synthesis

Spadin Chemical Structure

Spadin Chemical Structure

CAS No. : 1270083-24-3

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Based on 1 publication(s) in Google Scholar

Other Forms of Spadin:

Spadin Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Spadin

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5-HT1 Receptor 5-HT2 Receptor 5-HT3 Receptor 5-HT4 Receptor 5-HT5 Receptor 5-HT6 Receptor 5-HT7 Receptor 5-HT Receptor

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Description

Spadin, a natural peptide derived from a propeptide released in blood, is a potent TREK-1 channel blocker with IC50 value of 10 nM. Spadin enhances dorsal raphe nucleus 5-HT neurotransmission in mice and induces hippocampal CREB activation and neurogenesis. Spadin can be used for antidepressant research[1][2].

In Vitro

Spadin (100 nM; COS-7 cells) has inhibitory effect of spadin on the TREK-1 channel and blocks 63% of the TREK-1 current stimulated by arachidonic acid[1].
Spadin (100 nM) blocks the TREK-1 channels activity in CA3 hippocampal neurons on brain slices of wild-type mice[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Spadin Related Antibodies
In Vivo

Spadin (10 μM; i.p.; for 30 min; male C57Bl/6J and TREK-1 deficient mice) increases of the 5-HT neuron firing rate in the dorsal raphe nucleus (DRN)[2].
Spadin (0.01-100 μM; ICV, i.p. and i.v.; daily, for 7 days; male C57Bl/6J and TREK-1 deficient mice) has anti-depressant behavior in mice[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male C57Bl/6J and TREK-1 deficient mice[2]
Dosage: 10 μM
Administration: Intraperitoneal injection; for 30 min
Result: Increased of the 5-HT neuron firing rate in the dorsal raphe nucleus (DRN).
Animal Model: Male C57Bl/6J and TREK-1 deficient mice[2]
Dosage: 0.01-100 μM
Administration: Intracerebroventrical injection, intraperitoneal injection and intravenous injection; daily, for 7 days
Result: Had any effect on mouse locomotion analyzed in short- or long-time after the drug injection.
Molecular Weight

2012.34

Formula

C96H142N26O22
CAS No.
Appearance

Solid

Color

White to off-white

Sequence Shortening

YAPLPRWSGPIGVSWGLR
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

H2O : ≥ 100 mg/mL (49.69 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 0.4969 mL 2.4847 mL 4.9693 mL
5 mM 0.0994 mL 0.4969 mL 0.9939 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 0.4969 mL 2.4847 mL 4.9693 mL 12.4233 mL
5 mM 0.0994 mL 0.4969 mL 0.9939 mL 2.4847 mL
10 mM 0.0497 mL 0.2485 mL 0.4969 mL 1.2423 mL
15 mM 0.0331 mL 0.1656 mL 0.3313 mL 0.8282 mL
20 mM 0.0248 mL 0.1242 mL 0.2485 mL 0.6212 mL
25 mM 0.0199 mL 0.0994 mL 0.1988 mL 0.4969 mL
30 mM 0.0166 mL 0.0828 mL 0.1656 mL 0.4141 mL
40 mM 0.0124 mL 0.0621 mL 0.1242 mL 0.3106 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Spadin Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Spadin1270083-24-3Potassium Channel5-HT ReceptorKcsASerotonin Receptor5-hydroxytryptamine ReceptorTREK-15-HTantidepressantCREBneurogenesisCOS-7 cellsInhibitorinhibitorinhibit

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