FHZ

Cat. No.: HY-U00440: FAQs
Data Sheet SDS COA Handling Instructions

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FHZ is a fluorescent probe.

For research use only. We do not sell to patients.

FHZ Chemical Structure

CAS No. : 1883737-63-0

Size	Price	Stock
5 mg	USD 220	Ask For Quote & Lead Time
10 mg	USD 370	Ask For Quote & Lead Time

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

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Description

FHZ is a fluorescent probe.

In Vitro

After loaded with probe FHZ and treated with HClO and H₂O₂/EDTA-Fe²⁺ in order, HeLa cells display the bright fluorescences from both cyan and green channels. FHZ can give out two different fluorescent signals in the presence of both ?OH and HClO, suggesting the synchronous discrimination of ?OH and HClO by a dual-channel detection model with two exciting wavelengths. Probe FHZ shows very high specificity to the detections of ?OH and HClO with the excitations at 410 and 490 nm, respectively. The probe FHZ can efficiently enter the cellular mitochondria and exhibit the differentiable/visual capabilities to the endogenous ?OH and HClO by the dual fluorescent responses^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Probe FHZ can rapidly be absorbed into the blood circulation system from the zebrafish intestine, and spread out whole zebrafish tissues, and keep its stability in the blood, organs and tissues in the absence of ROS. The probe can keep its stability in biological environments and only selectively react with ?OH and HClO species^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

478.49

Formula

C₂₆H₂₆N₂O₇

CAS No.

1883737-63-0

Appearance

Solid

Color

Light brown to brown

SMILES

O=C1N(N)C2(C3=C(OC4=C2C=CC(OCCOCCOCCO)=C4)C=C(O)C=C3)C5=C1C=CC=C5

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (208.99 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.0899 mL	10.4495 mL	20.8991 mL
5 mM	0.4180 mL	2.0899 mL	4.1798 mL
10 mM	0.2090 mL	1.0450 mL	2.0899 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 98.17%

Select Batch:

Data Sheet (273 KB) SDS (251 KB)

COA (247 KB) HNMR (176 KB) RP-HPLC (99 KB) LCMS (109 KB)

Handling Instructions (2659 KB)

References

[1]. Zhang R, et al. Real-Time Discrimination and Versatile Profiling of Spontaneous Reactive Oxygen Species in Living Organisms with a Single Fluorescent Probe. J Am Chem Soc. 2016 Mar 23;138(11):3769-78. [Content Brief]

Cell Assay ^[1]	HeLa cells and RAW264.7 macrophages are cultured in high glucose Dulbecco’s modified Eagle’s medium (DMEM) supplied with 10% fetal bovine serum (FBS) and 1% antibotics (penicillin and streptomycin) at 37°C in humidified incubator containing 5% CO₂. The cells are seeded into glass-bottomed dishes and cultured for 24 h. Subsequently, the cells are incubated with FHZ for 30 min at 37°C and then washed with PBS buffer three times. Each treatment of cells with H₂O₂, EDTA-Fe²⁺, HClO or scavengers kept 30 min^[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration ^[1]	Wild type zebrafish is used in this study. Seven-day old fertilized zebrafish embryos are cultured in 50 μM FHZ for 30 min, and then the zebrafish embryos are transferred to fresh water. A FHZ-loaded zebrafish is fixed under confocal microscope using 2% agarose gel to keep its living state for fluorescent imaging. In order to observe the release of •OH in fresh wound, the ventral fin of the FHZ-loaded zebrafish is carefully cut a small wound using a blade. After raised for 20 min in water, the wound of injured zebrafish is imaged using confocal microscope^[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References	[1]. Zhang R, et al. Real-Time Discrimination and Versatile Profiling of Spontaneous Reactive Oxygen Species in Living Organisms with a Single Fluorescent Probe. J Am Chem Soc. 2016 Mar 23;138(11):3769-78. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.0899 mL	10.4495 mL	20.8991 mL	52.2477 mL
	5 mM	0.4180 mL	2.0899 mL	4.1798 mL	10.4495 mL
	10 mM	0.2090 mL	1.0450 mL	2.0899 mL	5.2248 mL
	15 mM	0.1393 mL	0.6966 mL	1.3933 mL	3.4832 mL
	20 mM	0.1045 mL	0.5225 mL	1.0450 mL	2.6124 mL
	25 mM	0.0836 mL	0.4180 mL	0.8360 mL	2.0899 mL
	30 mM	0.0697 mL	0.3483 mL	0.6966 mL	1.7416 mL
	40 mM	0.0522 mL	0.2612 mL	0.5225 mL	1.3062 mL
	50 mM	0.0418 mL	0.2090 mL	0.4180 mL	1.0450 mL
	60 mM	0.0348 mL	0.1742 mL	0.3483 mL	0.8708 mL
	80 mM	0.0261 mL	0.1306 mL	0.2612 mL	0.6531 mL
	100 mM	0.0209 mL	0.1045 mL	0.2090 mL	0.5225 mL

FHZ Related Classifications

Others

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

FHZ1883737-63-0Fluorescent DyeInhibitorinhibitorinhibit

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FHZ

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