Sulfo-CY5-NHS ester potassium (Synonyms: Sulfo-Cyanine5 NHS ester potassium)

Cat. No.: HY-135413A: FAQs
Data Sheet Handling Instructions

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MeCY5-NHS ester (potassium) is a reactive dye and can be used for labeling protein nucleic acid.

For research use only. We do not sell to patients.

Sulfo-CY5-NHS ester potassium Chemical Structure

CAS No. : 2230212-27-6

Size	Stock
5 mg	Get quote	Estimated Time of Arrival: December 31
10 mg	Get quote	Estimated Time of Arrival: December 31
25 mg	Get quote	Estimated Time of Arrival: December 31
50 mg	Get quote	Estimated Time of Arrival: December 31

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This product is a controlled substance and not for sale in your territory.

Other Forms of Sulfo-CY5-NHS ester potassium:

MeCY5-NHS ester triethylamine Get quote

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Biological Activity
Purity & Documentation
References
Customer Review

Description

MeCY5-NHS ester (potassium) is a reactive dye and can be used for labeling protein nucleic acid^[1].

Molecular Weight

777.95

Formula

C₃₆H₄₀KN₃O₁₀S₂

CAS No.

2230212-27-6

SMILES

O=C(CCC1=O)N1OC(CCCCC[N+]2=C(/C=C/C=C/C=C3C(C)(C4=CC(S(=O)(O[K])=O)=CC=C4N\3C)C)C(C)(C)C5=CC(S(=O)([O-])=O)=CC=C25)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent & Solubility

In Vitro:

DMSO : 62.5 mg/mL (80.34 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.2854 mL	6.4271 mL	12.8543 mL
5 mM	0.2571 mL	1.2854 mL	2.5709 mL
10 mM	0.1285 mL	0.6427 mL	1.2854 mL

View the Complete Stock Solution Preparation Table

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (2.67 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Shukla S,et al. Antibody Response against Cowpea Mosaic Viral Nanoparticles Improves In Situ Vaccine Efficacy in Ovarian Cancer. ACS Nano. 2020;14(3):2994-3003. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.2854 mL	6.4271 mL	12.8543 mL	32.1357 mL
	5 mM	0.2571 mL	1.2854 mL	2.5709 mL	6.4271 mL
	10 mM	0.1285 mL	0.6427 mL	1.2854 mL	3.2136 mL
	15 mM	0.0857 mL	0.4285 mL	0.8570 mL	2.1424 mL
	20 mM	0.0643 mL	0.3214 mL	0.6427 mL	1.6068 mL
	25 mM	0.0514 mL	0.2571 mL	0.5142 mL	1.2854 mL
	30 mM	0.0428 mL	0.2142 mL	0.4285 mL	1.0712 mL
	40 mM	0.0321 mL	0.1607 mL	0.3214 mL	0.8034 mL
	50 mM	0.0257 mL	0.1285 mL	0.2571 mL	0.6427 mL
	60 mM	0.0214 mL	0.1071 mL	0.2142 mL	0.5356 mL
	80 mM	0.0161 mL	0.0803 mL	0.1607 mL	0.4017 mL

Sulfo-CY5-NHS ester potassium Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Sulfo-CY5-NHS ester2230212-27-6Sulfo-Cyanine5 NHS esterDNA StainInhibitorinhibitorinhibit

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