Tween 80 (Synonyms: Polysorbate 80)

Name: Tween 80
Brand: MedChemExpress
SKU: HY-Y1891
Rating: 5.0 (56 reviews)

Cat. No.: HY-Y1891: FAQs
Data Sheet SDS COA Handling Instructions Technical Support

Tween 80 (Polysorbate 80), a surfactant, has been widely used as a solvent for pharmacological experiments. Tween 80 can also reduce bacterial attachment and inhibit biofilm formation.

For research use only. We do not sell to patients.

Tween 80 Chemical Structure

CAS No. : 9005-65-6

Size	Stock	Quantity
Solvent
50 mL	In-stock	Estimated Time of Arrival: December 31
Solvent
100 mL	In-stock	Estimated Time of Arrival: December 31
200 mL	Get quote
500 mL	Get quote

Synthetic products have potential research and development risk.

or Bulk Inquiry

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 56 publication(s) in Google Scholar

56 Publications Citing Use of MCE Tween 80

Featured Recommendations

•Related Small Molecules:

•Related Proteins:

Biological Activity
Purity & Documentation
References
Customer Review

Description

Tween 80 (Polysorbate 80), a surfactant, has been widely used as a solvent for pharmacological experiments. Tween 80 can also reduce bacterial attachment and inhibit biofilm formation.

In Vitro

Tween 80 (Polysorbate 80) is a surfactant which can also reduce bacterial attachment and inhibit biofilm formation^[1]. Solubility enhancement as high as 100-fold is achieved using 1% Tween 80 in vitro^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Tween 80 (Polysorbate 80) (IP; 10 ml/kg) significantly decreases locomotor activity at a concentration of 32% in saline (v/v) in male outbred (BALB/c x DBA/Z)F1 mice weighing 24-30 g^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

1309.63

Formula

C₆₄H₁₂₄O₂₆

CAS No.

9005-65-6

Appearance

Liquid (Density: 1.1 g/cm³)

Color

Colorless to light yellow

SMILES

[Tween 80]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Pure form	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : ≥ 100 mg/mL (76.36 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 50 mg/mL (38.18 mM; Need ultrasonic)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	0.7636 mL	3.8179 mL	7.6357 mL
5 mM	0.1527 mL	0.7636 mL	1.5271 mL
10 mM	0.0764 mL	0.3818 mL	0.7636 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (1.91 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (1.91 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (1.91 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Select Batch:

Data Sheet (272 KB) SDS (393 KB)

COA (246 KB)

Handling Instructions (2659 KB)

References

[1]. Brandl MT, et al. Effect of the surfactant tween 80 on the detachment and dispersal of Salmonella enterica serovar Thompson single cells and aggregates from cilantro leaves as revealed by image analysis. Appl Environ Microbiol. 2014 Aug;80(16):5037-42. [Content Brief]

[2]. Pan Y, et al. Pharmacokinetics study of ferulic acid in rats after oral administration of γ-oryzanol under combined use of Tween 80 by LC/MS/MS. Eur Rev Med Pharmacol Sci. 2014;18(2):143-50. [Content Brief]

[3]. C A Castro, et al.Behavioral effects of vehicles: DMSO, ethanol, Tween-20, Tween-80, and emulphor-620. Pharmacol Biochem Behav. 1995 Apr;50(4):521-6. [Content Brief]

[4]. Varma RK, Kaushal R, Junnarkar AY, et al. Polysorbate 80: a pharmacological study. Arzneimittelforschung. 1985;35(5):804-808. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	0.7636 mL	3.8179 mL	7.6357 mL	19.0894 mL
	5 mM	0.1527 mL	0.7636 mL	1.5271 mL	3.8179 mL
	10 mM	0.0764 mL	0.3818 mL	0.7636 mL	1.9089 mL
	15 mM	0.0509 mL	0.2545 mL	0.5090 mL	1.2726 mL
	20 mM	0.0382 mL	0.1909 mL	0.3818 mL	0.9545 mL
	25 mM	0.0305 mL	0.1527 mL	0.3054 mL	0.7636 mL
	30 mM	0.0255 mL	0.1273 mL	0.2545 mL	0.6363 mL
DMSO	40 mM	0.0191 mL	0.0954 mL	0.1909 mL	0.4772 mL
	50 mM	0.0153 mL	0.0764 mL	0.1527 mL	0.3818 mL
	60 mM	0.0127 mL	0.0636 mL	0.1273 mL	0.3182 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Tween 80 Related Classifications

Others

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Tween 809005-65-6Polysorbate 80Tween80Tween-80Polysorbate80Polysorbate-80Biochemical Assay ReagentsInhibitorinhibitorinhibit

Your Recently Viewed Products:

Product Name

Salutation

Applicant Name *

Email Address *

Phone Number *

Organization Name *

Department *

Requested quantity *

Country or Region *

Remarks

Product Name

Lot #

Applicant Name *

Email Address *

Phone Number

Department *

Organization Name *

Country or Region *

Remarks

Name
Email *

	Sorry, but the email address you supplied was invalid.

Tween 80 (Synonyms: Polysorbate 80)

Customer Review

Tween 80 Related Antibodies

56 Publications Citing Use of MCE Tween 80

Featured Recommendations

•Related Small Molecules:

•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

Tween 80 Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Tween 80 Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report