Tween 80 (Synonyms: Polysorbate 80)

Name: Tween 80
Brand: MedChemExpress
SKU: HY-Y1891
Rating: 5.0 (54 reviews)

Cat. No.: HY-Y1891: FAQs
Data Sheet SDS COA Handling Instructions

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Tween 80 (Polysorbate 80), a surfactant, has been widely used as a solvent for pharmacological experiments. Tween 80 can also reduce bacterial attachment and inhibit biofilm formation.

For research use only. We do not sell to patients.

Tween 80 Chemical Structure

CAS No. : 9005-65-6

Size	Price	Stock	Quantity
Solvent
50 mL	USD 50	In-stock	Estimated Time of Arrival: December 31
Solvent
100 mL	USD 57	In-stock	Estimated Time of Arrival: December 31
200 mL		Get quote
500 mL		Get quote

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Based on 54 publication(s) in Google Scholar

54 Publications Citing Use of MCE Tween 80

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Description

Tween 80 (Polysorbate 80), a surfactant, has been widely used as a solvent for pharmacological experiments. Tween 80 can also reduce bacterial attachment and inhibit biofilm formation.

In Vitro

Tween 80 (Polysorbate 80) is a surfactant which can also reduce bacterial attachment and inhibit biofilm formation^[1]. Solubility enhancement as high as 100-fold is achieved using 1% Tween 80 in vitro^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Tween 80 (Polysorbate 80) (IP; 10 ml/kg) significantly decreases locomotor activity at a concentration of 32% in saline (v/v) in male outbred (BALB/c x DBA/Z)F1 mice weighing 24-30 g^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

CAS No.

9005-65-6

Appearance

Liquid (Density: 1.1 g/cm³)

Color

Colorless to light yellow

SMILES

[Tween 80]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Pure form	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : ≥ 100 mg/mL

H₂O : 50 mg/mL (Need ultrasonic)

*"≥" means soluble, but saturation unknown.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL; Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL; Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL; Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Select Batch:

Data Sheet (272 KB) SDS (393 KB)

COA (306 KB)

Handling Instructions (2659 KB)

References

[1]. Brandl MT, et al. Effect of the surfactant tween 80 on the detachment and dispersal of Salmonella enterica serovar Thompson single cells and aggregates from cilantro leaves as revealed by image analysis. Appl Environ Microbiol. 2014 Aug;80(16):5037-42. [Content Brief]

[2]. Pan Y, et al. Pharmacokinetics study of ferulic acid in rats after oral administration of γ-oryzanol under combined use of Tween 80 by LC/MS/MS. Eur Rev Med Pharmacol Sci. 2014;18(2):143-50. [Content Brief]

[3]. C A Castro, et al.Behavioral effects of vehicles: DMSO, ethanol, Tween-20, Tween-80, and emulphor-620. Pharmacol Biochem Behav. 1995 Apr;50(4):521-6. [Content Brief]

[4]. Varma RK, Kaushal R, Junnarkar AY, et al. Polysorbate 80: a pharmacological study. Arzneimittelforschung. 1985;35(5):804-808. [Content Brief]

Tween 80 Related Classifications

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Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Tween 809005-65-6Polysorbate 80Tween80Tween-80Polysorbate80Polysorbate-80Biochemical Assay ReagentsInhibitorinhibitorinhibit

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Tween 80 (Synonyms: Polysorbate 80)

Customer Review

Tween 80 Related Antibodies

54 Publications Citing Use of MCE Tween 80

Featured Recommendations

•Related Small Molecules:

•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

Tween 80 Related Antibodies

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Tween 80 Related Classifications

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