21-Hydroxypregnenolone

Name: 21-Hydroxypregnenolone
Brand: MedChemExpress
SKU: HY-113020
Rating: 4.5 (1 reviews)

Cat. No.: HY-113020 Purity: 98.75%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

21-Hydroxypregnenolone is an essential intermediate in corticosterone synthesis.

For research use only. We do not sell to patients.

21-Hydroxypregnenolone Chemical Structure

CAS No. : 1164-98-3

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 88	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 88	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 51	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 80	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

1 Publications Citing Use of MCE 21-Hydroxypregnenolone

•Cell Metab. 2024 Oct 4:S1550-4131(24)00371-1. [Abstract]

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Biological Activity
Purity & Documentation
References
Customer Review

Description

21-Hydroxypregnenolone is an essential intermediate in corticosterone synthesis.

IC₅₀ & Target

Human Endogenous Metabolite

Molecular Weight

332.48

Formula

C₂₁H₃₂O₃

CAS No.

1164-98-3

Appearance

Solid

Color

White to off-white

SMILES

C[C@]12[C@]([H])([C@]3([C@]([H])(CC2)[C@]4(C)C(C[C@H](CC4)O)=CC3)[H])CC[C@@H]1C(CO)=O

Structure Classification

Steroids

Initial Source

Endogenous metabolite

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : ≥ 25 mg/mL (75.19 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.0077 mL	15.0385 mL	30.0770 mL
5 mM	0.6015 mL	3.0077 mL	6.0154 mL
10 mM	0.3008 mL	1.5038 mL	3.0077 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2 mg/mL (6.02 mM); Clear solution

This protocol yields a clear solution of ≥ 2 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2 mg/mL (6.02 mM); Clear solution

This protocol yields a clear solution of ≥ 2 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.75%

Select Batch:

Data Sheet (272 KB) SDS (251 KB)

COA (250 KB) HNMR (274 KB) LCMS (88 KB)

Handling Instructions (2659 KB)

References

[1]. Iudaev NA, et al. Pathways of corticosteroid biosynthesis in human adrenals (Itsenko-Cushing disease). Biokhimiia. 1976 Sep;41(9):1619-27. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.0077 mL	15.0385 mL	30.0770 mL	75.1925 mL
	5 mM	0.6015 mL	3.0077 mL	6.0154 mL	15.0385 mL
	10 mM	0.3008 mL	1.5038 mL	3.0077 mL	7.5192 mL
	15 mM	0.2005 mL	1.0026 mL	2.0051 mL	5.0128 mL
	20 mM	0.1504 mL	0.7519 mL	1.5038 mL	3.7596 mL
	25 mM	0.1203 mL	0.6015 mL	1.2031 mL	3.0077 mL
	30 mM	0.1003 mL	0.5013 mL	1.0026 mL	2.5064 mL
	40 mM	0.0752 mL	0.3760 mL	0.7519 mL	1.8798 mL
	50 mM	0.0602 mL	0.3008 mL	0.6015 mL	1.5038 mL
	60 mM	0.0501 mL	0.2506 mL	0.5013 mL	1.2532 mL