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  3. BTL-105

BTL-105 is a monobiotinylated Phos-tag derivative for the detection of phosphopeptides and phosphoproteins.

For research use only. We do not sell to patients.

BTL-105 Chemical Structure

BTL-105 Chemical Structure

CAS No. : 945007-11-4

Size Price Stock
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
USD 581 Ask For Quote & Lead Time
Solution
10 mM * 1 mL in DMSO USD 581 Ask For Quote & Lead Time
Solid
5 mg USD 380 Ask For Quote & Lead Time
10 mg USD 600 Ask For Quote & Lead Time
25 mg USD 1150 Ask For Quote & Lead Time
50 mg USD 1800 Ask For Quote & Lead Time
100 mg USD 2850 Ask For Quote & Lead Time

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Description

BTL-105 is a monobiotinylated Phos-tag derivative for the detection of phosphopeptides and phosphoproteins[1].

Molecular Weight

880.11

Formula

C46H61N11O5S

CAS No.
Appearance

Solid

Color

Off-white to light yellow

SMILES

O=C(NCCCCCC(NCCNC(C1=CC=C(CN(CC(O)CN(CC2=NC=CC=C2)CC3=NC=CC=C3)CC4=NC=CC=C4)N=C1)=O)=O)CCCC[C@@H]5SC[C@]([C@]5([H])N6)([H])NC6=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (113.62 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.1362 mL 5.6811 mL 11.3622 mL
5 mM 0.2272 mL 1.1362 mL 2.2724 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (2.84 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (2.84 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.1362 mL 5.6811 mL 11.3622 mL 28.4055 mL
5 mM 0.2272 mL 1.1362 mL 2.2724 mL 5.6811 mL
10 mM 0.1136 mL 0.5681 mL 1.1362 mL 2.8406 mL
15 mM 0.0757 mL 0.3787 mL 0.7575 mL 1.8937 mL
20 mM 0.0568 mL 0.2841 mL 0.5681 mL 1.4203 mL
25 mM 0.0454 mL 0.2272 mL 0.4545 mL 1.1362 mL
30 mM 0.0379 mL 0.1894 mL 0.3787 mL 0.9469 mL
40 mM 0.0284 mL 0.1420 mL 0.2841 mL 0.7101 mL
50 mM 0.0227 mL 0.1136 mL 0.2272 mL 0.5681 mL
60 mM 0.0189 mL 0.0947 mL 0.1894 mL 0.4734 mL
80 mM 0.0142 mL 0.0710 mL 0.1420 mL 0.3551 mL
100 mM 0.0114 mL 0.0568 mL 0.1136 mL 0.2841 mL
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BTL-105 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
BTL-105
Cat. No.:
HY-129780
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