2. Academic Validation
  3. Identification of a novel tumor suppressor gene p34 on human chromosome 6q25.1

Identification of a novel tumor suppressor gene p34 on human chromosome 6q25.1

  • Cancer Res. 2007 Jan 1;67(1):93-9. doi: 10.1158/0008-5472.CAN-06-2723.
Min Wang 1,  Haris G Vikis,  Yian Wang,  Dongmei Jia,  Daolong Wang,  Laura J Bierut,  Joan E Bailey-Wilson,  Christopher I Amos,  Susan M Pinney,  Gloria M Petersen,  Mariza de Andrade,  Ping Yang,  Jonathan S Wiest,  Pamela R Fain,  Ann G Schwartz,  Adi Gazdar,  John Minna,  Colette Gaba,  Henry Rothschild,  Diptasri Mandal,  Elena Kupert,  Daniela Seminara,  Yan Liu,  Avinash Viswanathan,  Ramaswamy Govindan,  Marshall W Anderson,  Ming You
Affiliations

Affiliation

  • 1 School of Medicine, Washington University, 660 Euclid Avenue, St. Louis, MO, USA.
PMID: 17210687 DOI: 10.1158/0008-5472.CAN-06-2723
Abstract

In this study, we observed loss of heterozygosity (LOH) in human chromosomal fragment 6q25.1 in sporadic lung Cancer patients. LOH was observed in 65% of the 26 lung tumors examined and was narrowed down to a 2.2-Mb region. Single-nucleotide polymorphism (SNP) analysis of genes located within this region identified a candidate gene, termed p34. This gene, also designated as ZC3H12D, C6orf95, FLJ46041, or dJ281H8.1, carries an A/G nonsynonymous SNP at codon 106, which alters the amino acid from lysine to arginine. Nearly 73% of heterozygous lung Cancer tissues with LOH and the A/G SNP also exhibited loss of the A allele. In vitro clonogenic and in vivo nude mouse studies showed that overexpression of the A allele exerts tumor suppressor function compared with the G allele. p34 is located within a recently mapped human lung Cancer susceptibility locus, and association of the p34 A/G SNP was tested among these families. No significant association between the less frequent G allele and lung Cancer susceptibility was found. Our results suggest that p34 may be a novel tumor suppressor gene involved in sporadic lung Cancer but it seems not to be the candidate familial lung Cancer susceptibility gene linked to chromosomal region 6q23-25.

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