Expression of human olfactory receptor 10J5 in heart aorta, coronary artery, and endothelial cells and its functional role in angiogenesis

Biochem Biophys Res Commun. 2015 May 1;460(2):404-8. doi: 10.1016/j.bbrc.2015.03.046.

Sung-Hee Kim ¹, Yeo Cho Yoon ¹, Ae Sin Lee ¹, NaNa Kang ², JaeHyung Koo ², Mee-Ra Rhyu ¹, Jae-Ho Park ³

Affiliations

1 Korea Food Research Institute, 1201-62 Anyangpangyo-ro, Bundang-gu, Seongnam-si, Gyeonggi-do 463-746, Republic of Korea.
2 Department of Brain Science, Daegu Gyeongbuk Institute of Science & Technology, Techno Jungang-daero, Hyeonpung-myeon, Dalseong-Gun, Daegu 711-873, Republic of Korea.
3 Korea Food Research Institute, 1201-62 Anyangpangyo-ro, Bundang-gu, Seongnam-si, Gyeonggi-do 463-746, Republic of Korea; Food Biotechnology, University of Science & Technology, 217 Gajeong-ro, Yuseong-gu, Daejeon 305-350, Republic of Korea. Electronic address: jaehopark@kfri.re.kr.

PMID: 25791473 DOI: 10.1016/j.bbrc.2015.03.046

Abstract

ORs are ectopically expressed in non-chemosensory tissues including muscle, kidney, and keratinocytes; however, their physiological roles are largely unknown. We found that human olfactory receptor 10J5 (OR10J5) is expressed in the human aorta, coronary artery, and umbilical vein endothelial cells (HUVEC). Lyral induces CA(2+) and phosphorylation of Akt in HUVEC. A knockdown study showed the inhibition of the lyral-induced CA(2+) and the phosphorylation Akt and implied that these processes are mediated by OR10J5. In addition, lyral enhanced migration of HUVEC, which were also inhibited by RNAi in a migration assay. In addition, matrigel plug assay showed that lyral enhanced angiogenesis in vivo. Together these data demonstrate the physiological role of OR10J5 in angiogenesis and represent roles of ORs in HUVEC cells.

Keywords

AKT; Angiogenesis; Calcium; HUVEC; OR10J5; Olfactory receptor.

Name
Email *

	Sorry, but the email address you supplied was invalid.