2. Academic Validation
  3. The pterocarpanquinone LQB 118 inhibits inflammation triggered by zymosan in vivo and in vitro

The pterocarpanquinone LQB 118 inhibits inflammation triggered by zymosan in vivo and in vitro

  • Int Immunopharmacol. 2020 Jun:83:106399. doi: 10.1016/j.intimp.2020.106399.
Éssia de Almeida Lima 1,  Luiz Henrique Agra Cavalcante-Silva 2,  Deyse Cristina Madruga Carvalho 1,  Chaquip Daher Netto 3,  Paulo Roberto Ribeiro Costa 4,  Sandra Rodrigues-Mascarenhas 5
Affiliations

Affiliations

  • 1 Biotechnology Center, Federal University of Paraíba, João Pessoa 58051‑900, Brazil.
  • 2 Health Science Center, Federal University of Paraíba, João Pessoa 58051‑900, Brazil.
  • 3 Laboratory of Chemistry, Federal University of Rio de Janeiro, 27930-560, Brazil.
  • 4 Laboratory of Bioorganic Chemistry, Natural Products Research Institute, Federal University of Rio de Janeiro, 21941-590, Brazil.
  • 5 Biotechnology Center, Federal University of Paraíba, João Pessoa 58051‑900, Brazil. Electronic address: sandra@cbiotec.ufpb.br.
PMID: 32193104 DOI: 10.1016/j.intimp.2020.106399
Abstract

LQB 118, a hydride molecule, has been described as an antineoplastic and antiparasitic drug. Recently, LQB118 was also shown to display anti-inflammatory properties using an LPS-induced lung inflammation model. However, LQB 118 effects on the inflammatory response induced by zymosan has not been demonstrated. In this study, swiss mice were LQB 118 intraperitoneally (i.p.) treated and zymosan was used to induce peritoneal inflammation. Peritoneal fluid was collected and used for cell counting and proinflammatory cytokines quantification (IL-1β, IL-6, and TNF-α) by immunoenzymatic assay (ELISA). For in vitro studies, peritoneal macrophages zymosan-stimulated were used. Results demonstrated that LQB 118 treatment reduced polymorphonuclear cell migration and TNF-α, IL-1β, and IL-6 levels in the peritoneal cavity. In macrophages, LQB 118 treatment display no cytotoxic effect and is also able to reduce cytokines levels. To investigate LQB 118 putative mechanism of action, TLR2, CD69, and P-p38 MAPK expression were evaluated. LQB 118 treatment reduced CD69 expression and p38 phosphorylation induced by zymosan. Furthermore, LQB 118 was able to negatively modulate TLR2 expression in the presence of inflammatory stimulus. Thus, our study provide new evidences for the mechanisms related to the anti-inflammatory effect of LQB 118 in vivo and in vitro.

Keywords

Cytokines; Inflammation; Macrophages; Zymosan.

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