2. Academic Validation
  3. Cloning of a human ether-a-go-go potassium channel expressed in myoblasts at the onset of fusion

Cloning of a human ether-a-go-go potassium channel expressed in myoblasts at the onset of fusion

  • FEBS Lett. 1998 Aug 28;434(1-2):177-82. doi: 10.1016/s0014-5793(98)00973-9.
T Occhiodoro 1,  L Bernheim,  J H Liu,  P Bijlenga,  M Sinnreich,  C R Bader,  J Fischer-Lougheed
Affiliations

Affiliation

  • 1 Département de Physiologie, Centre Médical Universitaire, Hôpital Cantonal Universitaire, Switzerland.
PMID: 9738473 DOI: 10.1016/s0014-5793(98)00973-9
Abstract

An early sign of human myoblast commitment to fusion is the expression of a non-inactivating delayed rectifier K+ current, I(K(NI)), and an associated membrane potential hyperpolarization. We have isolated the full-length coding region of a human ether-a-go-go K+ channel (h-eag) from myoblasts undergoing differentiation. The h-eag gene was localized to chromosome 1q32-41, and is expressed as a approximately 9 kb transcript in myogenic cells and in adult brain tissue. Forced expression of h-eag in undifferentiated myoblasts generates a current with remarkable similarity to I(K(NI)) indicating that h-eag constitutes the channel responsible for this current in vivo.

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