PHOSPHO1 Protein, Human (His)

N-His;Accession#Q8TCT1-1 PHOSPHO1 (M1-C267)

PHOSPHO1 Protein is a phosphatase with high activity toward phosphoethanolamine (PEA) and phosphocholine (PCho), playing a crucial role in the generation of inorganic phosphate for bone mineralization. It collaborates with PHOSPHO1 in skeletal mineralization, where PHOSPHO1 initiates hydroxyapatite crystallization in matrix vesicles (MVs), and ALPL/TNAP catalyzes the spread of hydroxyapatite crystallization in the extracellular matrix.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

N-His

Q8TCT1-1 (M1-C267)

162466  [NCBI]

MSGCFPVSGLRCLSRDGRMAAQGAPRFLLTFDFDETIVDENSDDSIVRAAPGQRLPESLRATYREGFYNEYMQRVFKYLGEQGVRPRDLSAIYEAIPLSPGMSDLLQFVAKQGACFEVILISDANTFGVESSLRAAGHHSLFRRILSNPSGPDARGLLALRPFHTHSCARCPANMCKHKVLSDYLRERAHDGVHFERLFYVGDGANDFCPMGLLAGGDVAFPRRGYPMHRLIQEAQKAEPSSFRASVVPWETAADVRLHLQQVLKSC

Approximately 32 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, 300mM L-Arginine, pH 7.0 or 20 mM PB, 200 mM NaCl, pH 8.0, 10% Glycerol.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.