SIRP alphaV8/CD172a Protein, Human (Biotinylated, HEK293, His-Avi)

SIRP alphaV8/CD172a Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived SIRP alphaV8/CD172a protein, expressed by HEK293 , with C-Avi, C-His labeled tag. The total length of SIRP alphaV8/CD172a Protein, Human (Biotinylated, HEK293, His-Avi) is 339 a.a., with molecular weight of 50-60 kDa.

Immobilized Human CD47 mFc at 2 μg/mL (100 μL/well) can bind Biotinylated Human SIRP alphaV8 His-Avi with a linear range of 0.02-1.25 μg/mL.

Human

HEK293

C-Avi;C-His

ATD50864 (E31-R369, S44L, S50T, I52T, H54R, V57A)

/

50-60 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized a 0.22 μm filtered solution of 20 mM Tris-HC1, 0.5 M NaCl, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.