2,4-Dihydroxypyrimidine-5-carboxylic Acid

Name: 2,4-Dihydroxypyrimidine-5-carboxylic Acid
Brand: MedChemExpress
SKU: HY-66047
Rating: 4.5 (1 reviews)

Cat. No.: HY-66047 Purity: 99.61%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

2,4-Dihydroxypyrimidine-5-carboxylic Acid is an endogenous metabolite.

For research use only. We do not sell to patients.

2,4-Dihydroxypyrimidine-5-carboxylic Acid Chemical Structure

CAS No. : 23945-44-0

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 39	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 39	In-stock	Estimated Time of Arrival: December 31
Solid
500 mg	USD 35	In-stock	Estimated Time of Arrival: December 31
1 g	USD 45	In-stock	Estimated Time of Arrival: December 31
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Biological Activity
Purity & Documentation
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Description

2,4-Dihydroxypyrimidine-5-carboxylic Acid is an endogenous metabolite.

Molecular Weight

156.10

Formula

C₅H₄N₂O₄

CAS No.

23945-44-0

Appearance

Solid

Color

White to light yellow

SMILES

O=C(N1)NC=C(C(O)=O)C1=O

Structure Classification

Ketones, Aldehydes, Acids

Initial Source

Endogenous metabolite

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 25 mg/mL (160.15 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 0.12 mg/mL (0.77 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	6.4061 mL	32.0308 mL	64.0615 mL
5 mM	1.2812 mL	6.4061 mL	12.8123 mL
10 mM	0.6406 mL	3.2031 mL	6.4061 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.75 mg/mL (17.62 mM); Clear solution

This protocol yields a clear solution of ≥ 2.75 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (27.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.75 mg/mL (17.62 mM); Clear solution

This protocol yields a clear solution of ≥ 2.75 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (27.5 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.61%

Select Batch:

Data Sheet (245 KB) SDS (393 KB)

COA (251 KB) HNMR (121 KB) RP-HPLC (260 KB)

Handling Instructions (2659 KB)

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	6.4061 mL	32.0307 mL	64.0615 mL	160.1537 mL
	5 mM	1.2812 mL	6.4061 mL	12.8123 mL	32.0307 mL
	10 mM	0.6406 mL	3.2031 mL	6.4061 mL	16.0154 mL
	15 mM	0.4271 mL	2.1354 mL	4.2708 mL	10.6769 mL
	20 mM	0.3203 mL	1.6015 mL	3.2031 mL	8.0077 mL
	25 mM	0.2562 mL	1.2812 mL	2.5625 mL	6.4061 mL
	30 mM	0.2135 mL	1.0677 mL	2.1354 mL	5.3385 mL
	40 mM	0.1602 mL	0.8008 mL	1.6015 mL	4.0038 mL
	50 mM	0.1281 mL	0.6406 mL	1.2812 mL	3.2031 mL
	60 mM	0.1068 mL	0.5338 mL	1.0677 mL	2.6692 mL
	80 mM	0.0801 mL	0.4004 mL	0.8008 mL	2.0019 mL
	100 mM	0.0641 mL	0.3203 mL	0.6406 mL	1.6015 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.