3-Methylpyrazole

Name: 3-Methylpyrazole
Brand: MedChemExpress
SKU: HY-66054
Rating: 4.5 (1 reviews)

Cat. No.: HY-66054 Purity: 99.54%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

3-Methylpyrazole is used as a nitrification inhibitor of nitrification in soil.

For research use only. We do not sell to patients.

3-Methylpyrazole Chemical Structure

CAS No. : 1453-58-3

Size	Price	Stock	Quantity
Solid or liquid + Solvent (Highly Recommended)
10 mM * 1 mL in Water ready for reconstitution	USD 28	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in Water	USD 28	In-stock	Estimated Time of Arrival: December 31
Solid or liquid
500 mg	USD 25	In-stock	Estimated Time of Arrival: December 31
1 g	USD 40	In-stock	Estimated Time of Arrival: December 31
5 g		Get quote
10 g		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
Customer Review

Description

3-Methylpyrazole is used as a nitrification inhibitor of nitrification in soil^[1].

In Vitro

3-Methylpyrazole could inhibit the metabolism of MAM by rat liver microsomes^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

82.10

Formula

C₄H₆N₂

CAS No.

1453-58-3

Appearance

<36.5°C Solid,>36.5°C Liquid

Color

Light yellow to reddish brown

SMILES

CC1=NNC=C1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Pure form	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

H₂O : 25 mg/mL (304.51 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	12.1803 mL	60.9013 mL	121.8027 mL
5 mM	2.4361 mL	12.1803 mL	24.3605 mL
10 mM	1.2180 mL	6.0901 mL	12.1803 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 50 mg/mL (609.01 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.54%

Select Batch:

Data Sheet (270 KB) SDS (779 KB)

COA (245 KB) RP-HPLC (246 KB)

Handling Instructions (2659 KB)

References

[1]. G.W. McCarty, et al. Inhibition of nitrification in soil by heterocyclic nitrogen compounds. Biol Fertil Soils (1989) 8:204-211.

[2]. Fiala ES, et al. Differential effects of 4-iodopyrazole and 3-methylpyrazole on the metabolic activation of methylazoxymethanol to a DNA methylating species by rat liver and rat colon mucosa in vivo. J Cancer Res Clin Oncol. 1987;113(2):145-50. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	12.1803 mL	60.9013 mL	121.8027 mL	304.5067 mL
	5 mM	2.4361 mL	12.1803 mL	24.3605 mL	60.9013 mL
	10 mM	1.2180 mL	6.0901 mL	12.1803 mL	30.4507 mL
	15 mM	0.8120 mL	4.0601 mL	8.1202 mL	20.3004 mL
	20 mM	0.6090 mL	3.0451 mL	6.0901 mL	15.2253 mL
	25 mM	0.4872 mL	2.4361 mL	4.8721 mL	12.1803 mL
	30 mM	0.4060 mL	2.0300 mL	4.0601 mL	10.1502 mL
	40 mM	0.3045 mL	1.5225 mL	3.0451 mL	7.6127 mL
	50 mM	0.2436 mL	1.2180 mL	2.4361 mL	6.0901 mL
	60 mM	0.2030 mL	1.0150 mL	2.0300 mL	5.0751 mL
	80 mM	0.1523 mL	0.7613 mL	1.5225 mL	3.8063 mL
	100 mM	0.1218 mL	0.6090 mL	1.2180 mL	3.0451 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.