Allocholic acid

Cat. No.: HY-125934: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Allocholic acid is a typically fetal bile acid found in vertebrates and reappears during liver regeneration and carcinogenesis, besides it is also a conjugate acid of allocholate and an isomer of cholic acid. Allocholic acid is a potent and specific stimulant of the adult olfactory system, it has a role as a marine metabolite, a rat metabolite and a human metabolite.

For research use only. We do not sell to patients.

Allocholic acid Chemical Structure

CAS No. : 2464-18-8

Size	Price	Stock
1 mg	USD 360	Ask For Quote & Lead Time
5 mg	USD 920	Ask For Quote & Lead Time
10 mg	USD 1480	Ask For Quote & Lead Time

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This product is a controlled substance and not for sale in your territory.

1 Publications Citing Use of MCE Allocholic acid

•Cancer Cell. 2024 Aug 12;42(8):1386-1400.e8. [Abstract]

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Biological Activity
Purity & Documentation
References
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Description

In Vitro

Allocholic acid induces the bai operon and stimulates the conversion of [24-¹⁴C] cholic acid to [24-¹⁴C] allodeoxycholic acid in cultures of C. scindens and C. hylemonae allodeoxycholic acid^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Allocholic acid is the major acidic biliary metabolite of cholestanol in the rat and in the gerbil^[5].
In the rat allolithocholic acid and allodeoxycholic acid undergo 7a-hydroxylation in the liver to provide allochenodeoxycholic and allocholic acids, respectively^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

408.57

Formula

C₂₄H₄₀O₅

CAS No.

2464-18-8

Appearance

Solid

Color

White to light yellow

SMILES

C[C@H](CCC(O)=O)[C@H]1CC[C@@]2([H])[C@]3([H])[C@H](O)C[C@@]4([H])C[C@H](O)CC[C@]4(C)[C@@]3([H])C[C@H](O)[C@]12C

Structure Classification

Steroids

Initial Source

Animals

Microorganisms

Duck gall

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 33.33 mg/mL (81.58 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.4476 mL	12.2378 mL	24.4756 mL
5 mM	0.4895 mL	2.4476 mL	4.8951 mL
10 mM	0.2448 mL	1.2238 mL	2.4476 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (5.09 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (5.09 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (5.09 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.76%

Select Batch:

Data Sheet (276 KB) SDS (251 KB)

COA (251 KB)

Handling Instructions (2659 KB)

References

[1]. ThomasBriggs, et al. Allocholic acid, the major component in bile from the river carpsucker, Carpiodes carpio (Rafinesque) (Catostomidae). Comp Biochem Physiol B. 1972 Jul 15;42(3):493-6. [Content Brief]

[2]. Li W, et al. The olfactory system of migratory adult sea lamprey (Petromyzon marinus) is specifically and acutely sensitive to unique bile acids released by conspecific larvae. J Gen Physiol. 1995 May;105(5):569-87. [Content Brief]

[3]. Mendoza ME, et al. Physiological characteristics of allo-cholic acid. J Lipid Res. 2003 Jan;44(1):84-92. [Content Brief]

[4]. Ridlon JM, et al. Isolation and characterization of a bile acid inducible 7alpha-dehydroxylating operon in Clostridium hylemonae TN271. Anaerobe. 2010 Apr;16(2):137-46. [Content Brief]

[5]. Elliott WH, Hyde PM. Metabolic pathways of bile acid synthesis. Am J Med. 1971 Nov;51(5):568-79. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.4476 mL	12.2378 mL	24.4756 mL	61.1890 mL
	5 mM	0.4895 mL	2.4476 mL	4.8951 mL	12.2378 mL
	10 mM	0.2448 mL	1.2238 mL	2.4476 mL	6.1189 mL
	15 mM	0.1632 mL	0.8159 mL	1.6317 mL	4.0793 mL
	20 mM	0.1224 mL	0.6119 mL	1.2238 mL	3.0595 mL
	25 mM	0.0979 mL	0.4895 mL	0.9790 mL	2.4476 mL
	30 mM	0.0816 mL	0.4079 mL	0.8159 mL	2.0396 mL
	40 mM	0.0612 mL	0.3059 mL	0.6119 mL	1.5297 mL
	50 mM	0.0490 mL	0.2448 mL	0.4895 mL	1.2238 mL
	60 mM	0.0408 mL	0.2040 mL	0.4079 mL	1.0198 mL
	80 mM	0.0306 mL	0.1530 mL	0.3059 mL	0.7649 mL

Allocholic acid Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Allocholic acid2464-18-8Othersvertebratesstimulantolfactorysystemfetalbilemetaboliteolfactory systemisomerInhibitorinhibitorinhibit

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