2. Cell Cycle/DNA Damage
  3. DNA Stain
  4. ​JOE

JOE is a fluorescent dye and can be used as a fluorescent label in DNA sequencing, PCR, and LCR amplifications. JOE possesses an absorbance of 525 nm and an emission of 550 nm.

For research use only. We do not sell to patients.

​JOE Chemical Structure

​JOE Chemical Structure

CAS No. : 82855-40-1

Size Price Stock Quantity
5 mg USD 25 In-stock
10 mg USD 36 In-stock
50 mg USD 113 In-stock
100 mg USD 180 In-stock
200 mg   Get quote  
500 mg   Get quote  

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Based on 1 publication(s) in Google Scholar

​JOE Related Antibodies

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Description

JOE is a fluorescent dye and can be used as a fluorescent label in DNA sequencing, PCR, and LCR amplifications. JOE possesses an absorbance of 525 nm and an emission of 550 nm[1].

Molecular Weight

505.26

Formula

C23H14Cl2O9
CAS No.
Appearance

Solid

Color

Brown to reddish brown

SMILES

O=C(C1=CC(C2(C3=C(OC4=C2C=C(OC)C(O)=C4Cl)C(Cl)=C(O)C(OC)=C3)O5)=C(C=C1)C5=O)O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (98.96 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 5 mg/mL (9.90 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.9792 mL 9.8959 mL 19.7918 mL
5 mM 0.3958 mL 1.9792 mL 3.9584 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 1.9792 mL 9.8959 mL 19.7918 mL 49.4795 mL
5 mM 0.3958 mL 1.9792 mL 3.9584 mL 9.8959 mL
DMSO 10 mM 0.1979 mL 0.9896 mL 1.9792 mL 4.9479 mL
15 mM 0.1319 mL 0.6597 mL 1.3195 mL 3.2986 mL
20 mM 0.0990 mL 0.4948 mL 0.9896 mL 2.4740 mL
25 mM 0.0792 mL 0.3958 mL 0.7917 mL 1.9792 mL
30 mM 0.0660 mL 0.3299 mL 0.6597 mL 1.6493 mL
40 mM 0.0495 mL 0.2474 mL 0.4948 mL 1.2370 mL
50 mM 0.0396 mL 0.1979 mL 0.3958 mL 0.9896 mL
60 mM 0.0330 mL 0.1649 mL 0.3299 mL 0.8247 mL
80 mM 0.0247 mL 0.1237 mL 0.2474 mL 0.6185 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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​JOE Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

​JOE82855-40-1DNA Stainfluorescent labelDNA sequencingPCRLCRInhibitorinhibitorinhibit

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