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  4. Fluorescent Dye

Fluorescent Dye

Fluorescence is a phenomenon of photoluminescence in luminescence. When an atom is irradiated by light, some electrons around the nucleus jump from their original orbitals to higher energy orbitals under the influence of light energy, i.e., from the ground state to the first or second excited single-line state, etc. Due to the instability of the first or second excited singlet state, the electron returns from the excited singlet state to the ground state accompanied by released energy, resulting in fluorescence.

Fluorescent dyes can be covalently bound or physically adsorbed in the structure of the substance. The fluorescence properties allow for the characterization, localization and quantitative analysis of the labeled object. Fluorescent dyes have shown great potential for the detection of proteins, nucleic acids, cells, and immunoassays, attributed to the advantages in non-radioactive contamination, simple experimental operation and easy observation.

Fluorescent dyes have penetrated many fields such as pharmacology, physiology, environmental science, and information science, as well as functional studies of proteins and drug screening.

Cat. No. Product Name Effect Purity Chemical Structure
  • HY-P1003
    Ac-DEVD-AMC
    		99.93%
    Ac-DEVD-AMC is a fluorescent substrate of caspase-3/caspase-7. When treating Ac-DEVD-AMC with cell lysate, Ac-DEVD-AMC releases amino-4-methylcoumarin (AMC) for fluorescence detection, with an excitation wavelength of 380 nm and an emission wavelength of 460 nm.
    Ac-DEVD-AMC
  • HY-15942
    5-TAMRA
    		99.25%
    5-TAMRA can produce bright, pH-insensitive orange-red fluorescence (excitation and emission extremes of 546/579) and has good photostability. 5-TAMRA is mainly used as a fluorescent marker for the synthesis and study of specific oligonucleotide probes.
    5-TAMRA
  • HY-P0019A
    Z-Gly-Gly-Arg-AMC acetate
    		99.87%
    Z-Gly-Gly-Arg-AMC acetate is a thrombin-specific fluorogenic substrate for testing of thrombin generation in PRP and platelet-poor plasma (PPP).
    Z-Gly-Gly-Arg-AMC acetate
  • HY-136626
    Z-Ala-Ala-Asn-AMC
    		99.47%
    Z-Ala-Ala-Asn-AMC (Cbz-Ala-Ala-Asn-AMC) is the legumain substrate. Overexpressed legumain in 293 HEK-Leg cells potently cleaved CBZ-Ala-Ala-Asn-AMC.
    Z-Ala-Ala-Asn-AMC
  • HY-D0799
    Sulfo-NHS-LC-Biotin sodium
    		98.32%
    Sulfo-NHS-LC-Biotin sodium is an amine-reactive biotinylation reagent that can be used for antibody labeling.
    Sulfo-NHS-LC-Biotin sodium
  • HY-D1730A
    AF488 NHS ester TEA
    AF488 NHS ester TEA is an amine specific fluorescence probe (λem=525 nm=525 nm). AF488 NHS ester reacts with sulfhydryl groups and amines in aqueous and biological samples then change their chemical structure and fluorescence properties after derivatization. AF488 exhibits the λem and λex wavelength of 520 nm and 470 nm, respectively.
    AF488 NHS ester TEA
  • HY-136886
    IR-820
    IR-820 (New Indocyanine Green) is an infrared blood pool contrast agent. IR-820 also is normally used as a laser and near-infrared dye to detect and quantify diseased tissue in live animals.
    IR-820
  • HY-D0915
    Brilliant Blue FCF
    		≥98.0%
    Brilliant Blue FCF is an aromatic hydrocarbon, a synthetic dye produced from petroleum and used as a colorant for food and other substances. The solution has a maximum absorption at 628 nm.
    Brilliant Blue FCF
  • HY-D0213
    Sudan Black B
    		99.13%
    Sudan Black B, a fat-soluble diazo dye, is a histochemical stain. Sudan Black B can be used for staining of neutral triglycerides and lipids.
    Sudan Black B
  • HY-101880
    ADHP
    		99.67%
    ADHP is a fluorogenic peroxidase substrate (λex=530 nm, λem=590 nm).
    ADHP
  • HY-15944
    5(6)-TAMRA
    		99.72%
    5(6)-TAMRA is a fluorescent dye molecule widely used as a label for peptides and proteins.
    5(6)-TAMRA
  • HY-D1069
    DBCO-Cy3
    		98.00%
    DBCO-Cy3 (DBCO-Sulfo-Cy3) is the derivative of Cyanine3 fluorophore, a pH insensitive from pH (4-10) orange fluorescent dyewith excitation maximum 555 nm and emission maximum of 580nm. DBCO-Cy3 has fast reaction kinetics and good stability, and is productive to use in many standard fluorescent instrumentations. DBCO-Cy3 is a click chemistry reagent, it contains a DBCO group that can undergo strain-promoted alkyne-azide cycloaddition (SPAAC) with molecules containing Azide groups.
    DBCO-Cy3
  • HY-D0236
    Congo Red
    Congo Red is an azo dye. Congo Red (CR) binding been used as a diagnostic test for the presence of amyloid in tissue sections.
    Congo Red
  • HY-137834
    Gly-Pro-AMC hydrobromide
    		98.68%
    Gly-Pro-AMC hydrobromide is a fluorescent dye, it can be used as a specific fluorescent substrate for detecting Dipeptidyl peptidase IV (DPP-IV) activity.
    Gly-Pro-AMC hydrobromide
  • HY-D0505
    Eosin Y
    		99.54%
    Eosin Y is a dye molecule. Eosin Y has low cost and good availability which has a wide application in organic synthesis as a photoredox catalyst.
    Eosin Y
  • HY-101895
    Fluorescein di(β-D-galactopyranoside)
    		99.81%
    Fluorescein di(β-D-galactopyranoside) is a fluorogenic substrate for β-galactosidase (λex=485 nm, λem=535 nm).
    Fluorescein di(β-D-galactopyranoside)
  • HY-D0933
    Auramine O
    Auramine O is a yellow fluorescent dye and can be used to stain acid-fast bacteria. Auramine O is toxic and resistant in the environment.
    Auramine O
  • HY-D1670
    Z-Gly-Pro-AMC
    		99.80%
    Z-Gly-Pro-AMC is a fluorogenic substrate. Z-Gly-Pro-AMC is hydrolyzed by prolyl endopeptidase to generate highly fluorescent 7-amido-4-methylcoumarin (HY-D0027). (λex=380 nm, λem=465 nm).
    Z-Gly-Pro-AMC
  • HY-P0028
    Phalloidin
    		99.83%
    Phalloidin is a mushroom-derived toxin which can be used to label F-actin of the cytoskeleton with fluorochrome .
    Phalloidin
  • HY-101891
    Di-8-ANEPPS
    		99.46%
    Di-8-ANEPPS is a naphthylstyryl voltage-sensitive dye, shifting both their fluorescence excitation and emission spectra upon changes in Vm .
    Di-8-ANEPPS
Cat. No. Product Name / Synonyms Application Reactivity