Z-Ala-Ala-Asn-AMC (Synonyms: Cbz-Ala-Ala-Asn-AMC)

Name: Z-Ala-Ala-Asn-AMC
Brand: MedChemExpress
SKU: HY-136626
Rating: 5.0 (3 reviews)

Cat. No.: HY-136626 Purity: 99.47%: FAQs
Data Sheet SDS COA Handling Instructions

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Z-Ala-Ala-Asn-AMC (Cbz-Ala-Ala-Asn-AMC) is the legumain substrate. Overexpressed legumain in 293 HEK-Leg cells potently cleaved CBZ-Ala-Ala-Asn-AMC.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

Z-Ala-Ala-Asn-AMC Chemical Structure

CAS No. : 149697-16-5

Size	Price	Stock	Quantity
1 mg	USD 140	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 340	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 630	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Based on 3 publication(s) in Google Scholar

3 Publications Citing Use of MCE Z-Ala-Ala-Asn-AMC

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Description

Z-Ala-Ala-Asn-AMC (Cbz-Ala-Ala-Asn-AMC) is the legumain substrate. Overexpressed legumain in 293 HEK-Leg cells potently cleaved CBZ-Ala-Ala-Asn-AMC^[1].

In Vitro

The first legumain fluorogenic substrate Cbz-Ala-Ala-Asn-AMC based on the P3-P2-P1 sequence Ala-Ala-Asn. The synthetic substrate Cbz-Ala-Ala-Asn-AMC is effectively cleaved by S. mansoni legumain and human legumain with K_ms of 90 and 80 uM respectively^[2]^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

565.57

Formula

C₂₈H₃₁N₅O₈

CAS No.

149697-16-5

Appearance

Solid

Color

White to off-white

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Sealed storage, away from moisture

Powder	-80°C	2 years
	-20°C	1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (88.41 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.7681 mL	8.8406 mL	17.6813 mL
5 mM	0.3536 mL	1.7681 mL	3.5363 mL
10 mM	0.1768 mL	0.8841 mL	1.7681 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (4.42 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (4.42 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (4.42 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.47%

Select Batch:

Data Sheet (271 KB) SDS (252 KB)

COA (245 KB) LCMS (87 KB) Elemental Analysis Report (104 KB)

Handling Instructions (2659 KB)

References

[1]. Stern L, Perry R, Ofek P, Many A, Shabat D, Satchi-Fainaro R. A novel antitumor prodrug platform designed to be cleaved by the endoprotease legumain. Bioconjug Chem. 2009;20(3):500-510. [Content Brief]

[2]. James C. Powers, et al. Propenoyl hydrazides. US7482379B2.

[3]. Poreba M, et al. Counter Selection Substrate Library Strategy for Developing Specific Protease Substrates and Probes. Cell Chem Biol. 2016;23(8):1023-1035. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.7681 mL	8.8406 mL	17.6813 mL	44.2032 mL
	5 mM	0.3536 mL	1.7681 mL	3.5363 mL	8.8406 mL
	10 mM	0.1768 mL	0.8841 mL	1.7681 mL	4.4203 mL
	15 mM	0.1179 mL	0.5894 mL	1.1788 mL	2.9469 mL
	20 mM	0.0884 mL	0.4420 mL	0.8841 mL	2.2102 mL
	25 mM	0.0707 mL	0.3536 mL	0.7073 mL	1.7681 mL
	30 mM	0.0589 mL	0.2947 mL	0.5894 mL	1.4734 mL
	40 mM	0.0442 mL	0.2210 mL	0.4420 mL	1.1051 mL
	50 mM	0.0354 mL	0.1768 mL	0.3536 mL	0.8841 mL
	60 mM	0.0295 mL	0.1473 mL	0.2947 mL	0.7367 mL
	80 mM	0.0221 mL	0.1105 mL	0.2210 mL	0.5525 mL

Z-Ala-Ala-Asn-AMC Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Z-Ala-Ala-Asn-AMC149697-16-5Cbz-Ala-Ala-Asn-AMCFluorescent DyelegumainsubstrateInhibitorinhibitorinhibit

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Z-Ala-Ala-Asn-AMC (Synonyms: Cbz-Ala-Ala-Asn-AMC)

Customer Review

Z-Ala-Ala-Asn-AMC Related Antibodies

3 Publications Citing Use of MCE Z-Ala-Ala-Asn-AMC

Featured Recommendations

•Related Small Molecules:

•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

Z-Ala-Ala-Asn-AMC Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Z-Ala-Ala-Asn-AMC Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

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