Phalloidin

Cat. No.: HY-P0028 Purity: 99.83%: FAQs
Data Sheet SDS COA Handling Instructions

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Phalloidin is a mushroom-derived toxin which can be used to label F-actin of the cytoskeleton with fluorochrome .

For research use only. We do not sell to patients.

Phalloidin Chemical Structure

CAS No. : 17466-45-4

Size	Price	Stock	Quantity
1 mg	USD 450	In-stock	Estimated Time of Arrival: December 31
5 mg		Get quote
10 mg		Get quote

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Based on 13 publication(s) in Google Scholar

13 Publications Citing Use of MCE Phalloidin

•SSRN. 2023 Nov 8.

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Description

Phalloidin is a mushroom-derived toxin which can be used to label F-actin of the cytoskeleton with fluorochrome ^[1].

In Vitro

Phalloidin staining will not work with methanol-fixed cells, probably because of the disruption of actin filament integrity, but it will work with cells fixed in 0.2% glutaraldehyde in PBS^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

788.87

Formula

C₃₅H₄₈N₈O₁₁S

CAS No.

17466-45-4

Appearance

Solid

Color

White to off-white

Sequence

Ala-{d-Thr}-Cys-{Hyp}-Ala-Trp-Leu (Sulfide bridge: Cys3-Trp6)

Sequence Shortening

A-{d-Thr}-C-{Hyp}-AWL (Sulfide bridge: Cys3-Trp6)

SMILES

[A-{d-Thr}-C-{Hyp}-AWL (Sulfide bridge: Cys3-Trp6)]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Sealed storage, away from moisture and light

Powder	-80°C	2 years
	-20°C	1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

DMSO : 10 mg/mL (12.68 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.2676 mL	6.3382 mL	12.6764 mL
5 mM	0.2535 mL	1.2676 mL	2.5353 mL
10 mM	0.1268 mL	0.6338 mL	1.2676 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

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μL

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.83%

Select Batch:

Data Sheet (270 KB) SDS (420 KB)

COA (242 KB) RP-HPLC (181 KB)

Handling Instructions (2659 KB)

References

[1]. Chazotte B. Labeling cytoskeletal F-actin with rhodamine phalloidin or fluorescein phalloidin for imaging. Cold Spring Harb Protoc. 2010 May;2010(5):pdb.prot4947. [Content Brief]

Cell Assay ^[1]	Prepare a 1:200 dilution of the stock solution (300 units/mL) of fluorescein Phalloidin or rhodamine Phalloidin using PBS. Aspirate the cell medium from the cells grown on glass coverslips and rinse the cells three times with PBS. Fix the cells for 10 min in the 3.7% formaldehyde solution. Rinse the fixed cells three times for 5 min each in PBS. Permeabilize the cells for 5 min in the 0.2% Triton X-100 solution. Rinse the fixed cells three times with PBS. Label the cells with the fluorescein or rhodamine Phalloidin for 5 to 10 min at room temperature. Rinse the cells three times in PBS for 5 min each time. Mount the cell and view the cells using either the fluorescein or rhodamine filter set, depending on the probe^[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References	[1]. Chazotte B. Labeling cytoskeletal F-actin with rhodamine phalloidin or fluorescein phalloidin for imaging. Cold Spring Harb Protoc. 2010 May;2010(5):pdb.prot4947. [Content Brief]

Cell Assay
^[1]

Prepare a 1:200 dilution of the stock solution (300 units/mL) of fluorescein Phalloidin or rhodamine Phalloidin using PBS. Aspirate the cell medium from the cells grown on glass coverslips and rinse the cells three times with PBS. Fix the cells for 10 min in the 3.7% formaldehyde solution. Rinse the fixed cells three times for 5 min each in PBS. Permeabilize the cells for 5 min in the 0.2% Triton X-100 solution. Rinse the fixed cells three times with PBS. Label the cells with the fluorescein or rhodamine Phalloidin for 5 to 10 min at room temperature. Rinse the cells three times in PBS for 5 min each time. Mount the cell and view the cells using either the fluorescein or rhodamine filter set, depending on the probe^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

[1]. Chazotte B. Labeling cytoskeletal F-actin with rhodamine phalloidin or fluorescein phalloidin for imaging. Cold Spring Harb Protoc. 2010 May;2010(5):pdb.prot4947. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.2676 mL	6.3382 mL	12.6764 mL	31.6909 mL
	5 mM	0.2535 mL	1.2676 mL	2.5353 mL	6.3382 mL
	10 mM	0.1268 mL	0.6338 mL	1.2676 mL	3.1691 mL

Phalloidin Related Classifications

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Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Phalloidin17466-45-4Fluorescent DyeInhibitorinhibitorinhibit

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Phalloidin

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Phalloidin Related Antibodies

13 Publications Citing Use of MCE Phalloidin

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•Related Small Molecules:

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Phalloidin Related Antibodies

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Complete Stock Solution Preparation Table

Phalloidin Related Classifications

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