2. Academic Validation
  3. Shizukaol B, an active sesquiterpene from Chloranthus henryi, attenuates LPS-induced inflammatory responses in BV2 microglial cells

Shizukaol B, an active sesquiterpene from Chloranthus henryi, attenuates LPS-induced inflammatory responses in BV2 microglial cells

  • Biomed Pharmacother. 2017 Apr;88:878-884. doi: 10.1016/j.biopha.2017.01.152.
Li-Long Pan 1,  Peng Xu 1,  Xiao-Ling Luo 1,  Li-Jun Wang 2,  Si-Yu Liu 1,  Yi-Zhun Zhu 1,  Jin-Feng Hu 3,  Xin-Hua Liu 4
Affiliations

Affiliations

  • 1 Shanghai Key Laboratory of Bioactive Small Molecules, Department of Pharmacology, School of Pharmacy, Fudan University, Shanghai 201203, China.
  • 2 Department of Natural Products Chemistry, School of Pharmacy, Fudan University, Shanghai 201203, China.
  • 3 Department of Natural Products Chemistry, School of Pharmacy, Fudan University, Shanghai 201203, China. Electronic address: jfhu@fudan.edu.cn.
  • 4 Shanghai Key Laboratory of Bioactive Small Molecules, Department of Pharmacology, School of Pharmacy, Fudan University, Shanghai 201203, China. Electronic address: liuxinhua@fudan.edu.cn.
PMID: 28178617 DOI: 10.1016/j.biopha.2017.01.152
Abstract

The objective of the current study was to evaluate the anti-inflammatory effects of shizukaol B, a lindenane-type dimeric sesquiterpene isolated from the whole plant of Chloranthus henryi, on lipopolysaccharide (LPS)-induced activation of BV2 microglial cells in vitro. Our data showed that shizukaol B concentration-dependently suppressed expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), production of nitric oxide (NO), tumor necrosis factor-α (TNF-α), and interleukin-1β (IL-1β) in LPS-stimulated BV2 microglia. Meanwhile, shizukaol B concentration- and time-dependently inhibited LPS-mediated c-Jun N-terminal kinase 1/2 (JNK) activation, but had little effect on extracellular signal-regulated kinase 1/2 or p38 phosphorylation. Furthermore, shizukaol B significantly blocked LPS-induced activator protein-1 (AP-1) activation, evidenced by reduced phosphorylation and nuclear translocation of c-Jun and DNA binding activity of AP-1. Taken together, our findings suggest that shizukaol B exerts anti-inflammatory effects in LPS-activated microglia partly by modulating JNK-AP-1 signaling pathway.

Keywords

Activator protein-1; BV2 microglia cells; Lipopolysaccharide; Neuro-inflammation; Shizukaol B.

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