2. Academic Validation
  3. Geraniin promotes osteoblast proliferation and differentiation via the activation of Wnt/β-catenin pathway

Geraniin promotes osteoblast proliferation and differentiation via the activation of Wnt/β-catenin pathway

  • Biomed Pharmacother. 2018 Mar;99:319-324. doi: 10.1016/j.biopha.2018.01.040.
Kejia Li 1,  Xiaochao Zhang 2,  Bo He 2,  Renhua Yang 2,  Yue Zhang 2,  Zhiqiang Shen 3,  Peng Chen 4,  Wei Du 5
Affiliations

Affiliations

  • 1 School of Pharmaceutical Sciences and Yunnan Key Laboratory of Pharmacology for Natural Products, Kunming Medical University, 1168 West Chunrong Road, Kunming, Yunnan 650500, China; Department of Pharmacy, The Second Affiliated Hospital of Kunming Medical University, 374 Dianmian Avenue, Kunming, Yunnan 650101, China.
  • 2 School of Pharmaceutical Sciences and Yunnan Key Laboratory of Pharmacology for Natural Products, Kunming Medical University, 1168 West Chunrong Road, Kunming, Yunnan 650500, China.
  • 3 School of Pharmaceutical Sciences and Yunnan Key Laboratory of Pharmacology for Natural Products, Kunming Medical University, 1168 West Chunrong Road, Kunming, Yunnan 650500, China. Electronic address: shenzhiqiang@kmmc.edu.cn.
  • 4 School of Pharmaceutical Sciences and Yunnan Key Laboratory of Pharmacology for Natural Products, Kunming Medical University, 1168 West Chunrong Road, Kunming, Yunnan 650500, China. Electronic address: chenpeng@kmmc.edu.cn.
  • 5 Department of Radiology, The First Affiliated Hospital of Dali University, 32 Carlsberg Avenue, Dali, Yunnan 671000, China. Electronic address: 2393350136@qq.com.
PMID: 29353207 DOI: 10.1016/j.biopha.2018.01.040
Abstract

Geraniin is an ellagitannin isolated from Phyllanthus amarus and has a wide range of bioactivities. Our previous study demonstrated that geraniin could alleviate osteoporosis by accelerating bone formation, but the mechanism remains unclear. This study aimed to elucidate the molecular mechanisms by which geraniin promotes osteoblast proliferation and differentiation in vitro. Primary rat bone marrow-derived mesenchymal stem cells were separated and divided into sham operated (Sham) group, Sham treated with geraniin (Sham + GE) group, ovariectomized (OVX) group, OVX treated with geraniin (OVX + GE) group, OVX treated with osteogenic medium (OVX + OM) group, OVX treated with Wnt Inhibitor (OVX + WI) group, and OVX treated with Wnt Inhibitor and geraniin (OVX + W I + GE) group. Following bilateral ovariectomy, the expression of β-catenin, frizzled2, LRP6, TCF4, LEF1, c-Myc, cyclin D1, Runx2 and osterix significantly reduced, while the expression of axin2 significantly increased (P < 0.05). Geraniin enhanced the expression of β-catenin, frizzled2, LRP6, TCF4, LEF1, c-Myc, cyclin D1, Runx2 and osterix, while inhibited the expression of axin2 (P < 0.05). Wnt Inhibitor significantly weakened geraniin-induced Wnt/β-catenin activation (P < 0.05). In conclusion, geraniin enhances the activation of Wnt/β-catenin pathway, which may explain how it promotes osteoblast proliferation and differentiation.

Keywords

Geraniin; Osteoblastic differentiation; Osteogenesis; Wnt/β-catenin pathway.

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