Drug Target Identification Service

SPIDER^TM Target Identification

Specific Pupylation as IDEntity Reporter (SPIDER) is a method for identifying interactions between proteins and biomolecules. This approach utilizes substrate proximity labeling activity in conjunction with the streptavidin (SA)-biotin system for detection.

In a typical SPIDER assay, when the target protein (Prey) interacts with a biotin-labeled biomolecule (Bait), it brought into proximity with the biotin-conjugated SAm-Pup^E. Under the catalytic action of the PafA enzyme, the C-terminus of Pup^E covalently links to lysine residues on the surface of the Prey protein, effectively capturing the target protein. This process converts the non-covalent interaction between the biomolecule and the protein into a covalent bond between SAm-Pup^E and the target protein, facilitating the stable enrichment, identification, and analysis of the target protein in subsequent steps. The enriched proteins are then identified using high-resolution mass spectrometry, revealing potential drug targets.

DARTS Target Identification

The Drug Affinity Responsive Target Stability (DARTS) technology is based on the principle that the stability of a target protein increases upon binding with small molecular ligands, thereby enhancing the protein's resistance to proteolytic degradation. This enhanced stability enables the selection and identification of target proteins using high-resolution mass spectrometry.

DARTS is capable of identifying targets for individual small molecules, as well as to discover the action targets of complex natural products or herbal formulations. This technology significantly improves the efficiency of target discovery and demonstrates exceptional screening performance for membrane proteins, cytoplasmic proteins, and nuclear proteins, all without the need for drug labeling.

SPR Target Identification

Surface Plasmon Resonance (SPR) is a label-free, real-time technology used to detect molecular interactions. It operates on the principle of surface plasmon resonance, enabling continuous monitoring of the binding and dissociation processes of molecules on a solid surface, typically a metal surface.

In this method, drugs are immobilized on a chip, and protein lysates are diluted to various concentrations. By applying these lysates at multiple concentrations, the interactions between the lysates and the drug can be assessed at different levels. Proteins that bind to the small molecules are collected and identified using high-resolution mass spectrometry, enabling the identification of potential drug target proteins.

Online Consulting

Drug target identification service requires an initial evaluation to determine the appropriate scheme and pricing. For additional information about service price and technical specifics, please send an email to sales@MedChemExpress.com or contact the sales staff of MedChemExpress directly.