2. Academic Validation
  3. AMOT130/YAP pathway in topography-induced BMSC osteoblastic differentiation

AMOT130/YAP pathway in topography-induced BMSC osteoblastic differentiation

  • Colloids Surf B Biointerfaces. 2019 Oct 1;182:110332. doi: 10.1016/j.colsurfb.2019.06.061.
Xuan Liu 1,  Wenqing Hou 1,  Lei He 2,  Fangping Han 3,  Mengjie Lu 4,  Xiaobo Lu 5,  Ke Duan 6,  Tailin Guo 7,  Jie Weng 2
Affiliations

Affiliations

  • 1 College of Medicine, Southwest Jiaotong University, Chengdu 610031, China.
  • 2 Key Laboratory of Advanced Technologies of Materials (MOE), School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China.
  • 3 School of Life Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China.
  • 4 Department of Pathology, Affiliated Hospital of Southwest Medical University, Luzhou 646000, China; Laboratory of Orthopaedic Engineering, Department of Bone and Joint Surgery, Affiliated Hospital of Southwest Medical University, Luzhou 646000, China.
  • 5 Laboratory of Orthopaedic Engineering, Department of Bone and Joint Surgery, Affiliated Hospital of Southwest Medical University, Luzhou 646000, China.
  • 6 Laboratory of Orthopaedic Engineering, Department of Bone and Joint Surgery, Affiliated Hospital of Southwest Medical University, Luzhou 646000, China. Electronic address: keduan9@126.com.
  • 7 College of Medicine, Southwest Jiaotong University, Chengdu 610031, China. Electronic address: tlguo@home.swjtu.edu.cn.
PMID: 31325776 DOI: 10.1016/j.colsurfb.2019.06.061
Abstract

Micro/nano-topography (MNT) is an important variable affecting osseointegration of bone biomaterials, but the underlying mechanisms are not fully understood. We probed the role of a AMOT130/YAP pathway in osteoblastic differentiation of bone marrow mesenchymal stems cultured on titanium (Ti) carrying MNTs. Ti surfaces with two well-defined MNTs (TiO2 nanotubes of different diameters and wall thicknesses) were prepared by anodization. Rat BMSCs were cultured on flat Ti and Ti surfaces carrying MNTs, and cell behaviors (i.e., morphology, F-actin development, osteoblastic differentiation, YAP localization) were studied. Ti surfaces carrying MNTs increased F-actin formation, osteoblastic gene expression, and protein AMOT130 production in BMSCs (all vs. flat Ti), and the surface carrying larger nantubes was more effective, confirming osteoblastic differentiation induced by MNTs. Elevation of the AMOT130 level (by inhibiting its degradation) increased the osteoblastic gene expression, F-actin formation, and nuclear localization of YAP. These show that, AMOT130/YAP is an important pathway mediating the translation of MNT signals to BMSC osteoblastic commitment, likely via the cascade: AMOT130 promotion of F-actin formation, increased YAP nuclear import, and activation of osteoblastic gene expression.

Keywords

AMOT130; Differentiation; Mechanotransduction; Micro/nano-topography; YAP.

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