2. Academic Validation
  3. Wnt-3a improves functional recovery after spinal cord injury by regulating the inflammatory and apoptotic response in rats via wnt/β-catenin signaling pathway

Wnt-3a improves functional recovery after spinal cord injury by regulating the inflammatory and apoptotic response in rats via wnt/β-catenin signaling pathway

  • Brain Res. 2023 Oct 17:148637. doi: 10.1016/j.brainres.2023.148637.
Kai Gao 1,  Wenbo Shao 2,  Tian Wei 3,  Zihan Yan 4,  Nianhu Li 5,  Chaoliang Lv 6
Affiliations

Affiliations

  • 1 Postdoctoral Shandong University of Traditional Chinese Medicine, Jinan, China; Department of Orthopedics, Jining No.1 People's Hospital, Jining, China.
  • 2 Department of Orthopedics, Jining No.2 People's Hospital, Jining, China.
  • 3 School of Clinical Medicine, Shandong University of Traditional Chinese Medicine, Jinan, China.
  • 4 School of Clinical Medicine, Jining Medical University, Jining, China.
  • 5 Postdoctoral Shandong University of Traditional Chinese Medicine, Jinan, China; Department of Orthopedics, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, China. Electronic address: tigerlee073@126.com.
  • 6 Department of Orthopedics, Jining No.1 People's Hospital, Jining, China; School of Clinical Medicine, Jining Medical University, Jining, China. Electronic address: lvchaolianggk@163.com.
PMID: 37858855 DOI: 10.1016/j.brainres.2023.148637
Abstract

The specific molecular mechanism of neuroprotective effects of wnt-3a on spinal cord injury (SCI) has not been elucidated. In our study, we evaluated the recovery of motor function after SCI by BBB, observed neuronal Apoptosis by western blot and TUNEL, observed the changes of neuronal inflammation by western blot and immunofluorescence staining, and observed the changes of motoneurons and spinal cord area in the anterior horn of the spinal cord via Nissl and HE staining. We found that wnt-3a could significantly promote the recovery of motor function, reduce the loss of motor neurons in the anterior horn of the spinal cord, promote the recovery of injured spinal cord tissue, inhibit neuronal Apoptosis and inflammatory response, and ultimately promote neuronal function after SCI. However, when XAV939 inhibits the Wnt/β-catenin signaling pathway, the neuroprotective effects of wnt-3a are also significantly inhibited. The above results together indicated that wnt-3a exerts its neuroprotective effect on after SCI via activating the Wnt/β-catenin signaling pathway.

Keywords

Apoptosis; Inflammation; Spinal cord injury; Wnt-3a; Wnt/β-catenin signaling pathway.

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